K-AGLUA
50 assays (manual) / 200 assays (microplate)
| Content: | 50 assays (manual) / 200 assays (microplate) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Glucuronidase |
| Assay Format: | Spectrophotometer, Microplate |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 17.4 to 174 mU/mL of α-D-glucuronidase per assay |
| Limit of Detection: | 17 mU/mL |
| Reaction Time (min): | ~ 25 min |
| Application examples: | Enzyme preparations and other materials. |
| Method recognition: | Novel method |
This product has been discontinued (read more).
The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.
New, improved substrate.
This kit contains highly purified, borohydride reduced aldotriouronic acid with a terminal α-D-glucuronic acid substitution, which is an excellent substrate for α-D-glucuronidases from GH67. This substrate can be also used for the measurement of α-D-glucuronidases from GH115, however the rate of hydrolysis compared to GH67 is reduced. Previously this kit contained a mixture of borohydride reduced aldouronic acids (tri:tetra:penta).
View more test kits for enzyme activity measurement.
Advantages
The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.
Control System:
5.4”LCD Touch Screen Display
>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.
>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.
>Ithas the functions of cold trap precooling and vacuum pump preheating,and the time can be set by the userin the system. >Display whether the current running status is normal or not by numerical color.
>Built-in saturated vapor pressure &temperature corresponding relationship table,dearly displaying the corresponding relationship.
o One-key operation,the operation is extremely simple,no special professional learning and training is required.
o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably.
o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.
o Ambient temperature monitoring,the device automatically reminds the alarm if the ambient temperature is too high.
o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.
Drying chamber | · The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional) |
Cold trap andcondensing coil | · The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation. |
Sample shelves | · 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf. |
Refrigerationsystem | · Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures. |
Techinical parameter
| Item No | ST7003 | ST7006 | ST9006 | |
| Ice condenser capacity | 3kg | 6kg | 6kg | |
| Ice condenser temperature | -70℃ | -70℃ | -90℃ | |
| Cold trap volume | 5L | 10L | 10L | |
| Freeze dried chamber | Bell shape (standard configuration)/Multi manifold type/T-shaped frame type | |||
| Sample shelf | φ200mm.Single layer area 0.03m2 with 3-5 layers available | ●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona | ||
| Vacuum pump | Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types | |||
| Oil mist filte | Standard configuration | |||
| Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | |||
| Anti-corrosive treatment | Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents. | |||
| External valve | 6pieces/optional | 1-48 pieces/optional | ||
| Eutectic point detection system | Support/Optional | |||
| Data output analysis | Support/Optional | |||
| Powe | 0.6kw | 1.1kw | 1.5kw | |
| Size | 420*545*455mm | 510*570*500mm | ||
Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006
Brand:
Yingtai
Magnetic Beads (tRNA Purification)
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.
Workflow without large RNA/DNA contamination
In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.
Workflow with large RNA/DNA contamination
Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.
Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).
Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.
Features
Removal of unwanted components and other impurities
Purification of tRNA and oligos (>70 nt)
tRNA
RNA fragments 70 nt or longer
DNA/RNA hybrid fragments 70 nt or longer
Oligo and chimeric oligo 70 nt or longer
dsDNA fragments 70 bp or longer
ssDNA fragments 70 nt or longer
Removal of larger RNA/DNA contamination:
18S rRNA
28S rRNA
RNA/DNA> 180 nt
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.