Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Figure 1 / 4
Click for expanded view
| Kit Specifications – Spin Column | |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi | 50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves | 60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications | 30 minutes |
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
| Component | Cat. 25800 (50 preps) | Cat. 31350 (100 preps) | Cat. 25850 (250 preps) | Cat. 31900 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer C | 60 mL | 1 x 30 mL, 1 x 60 mL | 3 x 60 mL | 2 x 60 mL |
| Wash Solution A | 38 mL | 38 mL | 1 x 18 mL, 2 x 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL | 20 mL |
| Filter Columns | 50 | 100 | 250 | – |
| Spin Columns | 50 | 100 | 250 | – |
| 96-Well Plate | – | – | – | 2 |
| Adhesive Tape | – | – | – | 4 |
| Collection Tubes | 100 | 200 | 500 | – |
| 96-Well Collection Plate | – | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | – |
| 96-Well Elution Plate | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
Mycoplasma is the smallest and simplest prokaryotic organism. There is a risk of mycoplasma contamination during cell culture, and mycoplasma contamination of cells has become a common problem worldwide.
Mycoplasma contamination may seriously affect the state of cells, change the gene expression and metabolic characteristics of cells, lead to slow cell growth, abnormal differentiation and death, and seriously affect cell function.
Bacteria, yeast or mold contamination in cell culture can be seen under an optical microscope, but mycoplasma contamination is usually not visible under an optical microscope and must be detected by specific detection methods.
Common methods for detecting mycoplasma contamination include mycoplasma isolation and culture, special biochemical tests such as ELISA and luminescence, and DNA fluorescent staining detection. Among the above detection methods, most of the operation steps are relatively cumbersome, the sensitivity is not high, the mycoplasma types cannot be distinguished, special instruments are required, or the time required is long. The qPCR method is relatively simple and convenient to operate, and the results can be obtained in 2 hours.
• Provide results in less than 2 hours.
Good negative and positive controls.
• No live mycoplasma required: The MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require the use of live mycoplasma for validation
• Can be used with gDNA, inactivated mycoplasma, or live mycoplasma
• Can be used as an alternative to the standard 28-day culture test
• Can be used in conjunction with culture-based methods to provide preliminary results while awaiting the 28-day test results
The MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious drug, impurity, and contaminant detection during biopharmaceutical manufacturing. The entire workflow includes the sample preparation kit, which provides a manual sample preparation method, which together with the qPCR analysis can provide results in 2 hours. (Please note that complex matrices may require additional upfront processing steps as described in the various available protocols.)
• Mycoplasma qPCR MIX, store at -15°C to -25°C, store at 2°C to 8°C after first use, protected from light.
• Mycoplasma Positive Control, -15°C to -25°C.
• Mycoplasma Negative Control, store at -15°C to -25°C, store at 2°C to 8°C after first use.
Note: Price not include shipment & duty, contact us to get full quote.
DuckyBio’s Mycoplasma PCR Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for detecting mycoplasma contamination in biological materials such as cultured cells. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in European Pharmacopoeia (E.P. 2.6.7, 2007), US Pharmacopoeia (US63) and Japanese Pharmacopoeia. When used with a suitable sample preparation method, the MycoSEQ Plus kit can detect less than 5 CFU/mL.
K-GLUHK-220A
SKU: 700004299
| Content: | (K-GLUHK-110A) 110 assays (manual) / 1100 assays (microplate) / 1000 assays (auto-analyser) (K-GLUHK-220A) 220 assays (manual) / 2200 assays (microplate) / 2000 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Glucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 µg of D-glucose per assay |
| Limit of Detection: | 0.66 mg/L |
| Reaction Time (min): | ~ 5 min |
| Application examples: | Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and ASBC Method Malt 6-D |
The D-Glucose HK (Regular) test kit is a high purity reagent for the measurement and analysis of D-glucose in plant and food products. Can be used in combination with other Megazyme’s products that require glucose determination.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Browse more of our monosaccharide and oligosaccharide assay kits.
Advantages