Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. Low-risk HPV types 6 and 11 have been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA. HPV 16 and HPV 18 have been considered as high-risk cancer associated HPV types. HPV types 31, 33, and 35 have been shown to have an intermediate relationship with cancer. Norgen’s Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits are suitable for the detection of HPV 33 and HPV 58.
HPV (High and Low Risk) TaqMan PCR Kit, 100 reactions
HPV (High and Low Risk) TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM31550 (100 preps) | Cat. TM31510 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HPV (High and Low Risk) Primer & Probe Mix | 280 μL | 280 μL |
| HPV (High and Low Risk) Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Attogene’s Microcystin Test Kit (Rapid – Drinking Water) can be used to detect microcystins in water to as low as 0.1ppb; highly sensitive, rapid, robust screening kit for microcystins and nodularins.
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on thresholds for microcytin-LR concentrations in drinking waters has been developed. This is guidance that allows states to take various actions.
For the rapid screening of microcystins in drinking water samples at or above 0.1 ppb. Samples requiring regulatory action should be confirmed by ELISA, HPLC, or other conventional methods.
To protect consumers from adverse health effects caused by these toxins, the World Health Organization (WHO) has proposed a provisional upper limit for Microcystin-LR of 1.0 ppb (μg/L) in drinking water.
The U.S. Environmental Protection Agency (EPA) has also established guidelines for Microcystins in drinking water:
-For children below school age, 0.3 μg/L (ppb)
-For all other age groups, 1.6 μg/L (ppb)
Screening of Microcystins in water samples at 0.1 ppb (drinking water)
Format: 10 tests (5 tests/5 controls)
Not provided: Water Sample Bottles
Run Time: 15 Minutes
Finished Drinking Water