Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
HL-ExoI is used for degradation of ssDNA such as primers and oligos. It is also ideal for treatment of sensitive samples and useful in the development of novel molecular diagnostics applications.
Removal of primers post-PCR prior to DNA sequencing or SNP detection
Quality Control
ArcticZymes is dedicated to the quality of our products. We manufacture all products at our ISO 13485 certified facility in Norway.
Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
Norgen’s Legionella sp. TaqMan PCR Lyophilized Kit is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Legionella sp. TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Figure 1 / 3
Click for expanded view
| Component | Cat. TM64450L (100 preps) | Cat. TM64410L (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix (Lyo) | 4 x 350 μL | – |
| Legionella sp. Primer & Probe Mix (Lyo) | 280 μL | 280 μL |
| Legionella sp. Positive Control (Lyo) | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 3 x 1.25 mL | 1 x 1.25 mL |
| Product Insert | 1 | 1 |
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately. This Kit combines phenol/guanidine-based lysis of samples and silica membrane–based purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
Specifications
| Features | Specifications |
| Main Functions | Isolation miRNA and other small RNA molecules(18nt), from cultured cells and various animal and human tissues, using MagZol reagent and column |
| Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissues, adherent cells, suspension cells, bacteria, etc |
| Sample amount | Eukaryotic culture cells: ≤ 10^7, Animal tissue:<100mg, Yeast culture cells:<5 x10^7, Bacteria:<10^9 |
| Elution volume | ≥15μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such asguanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein andother impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
This kit combines acid/guanidine (MagZol) extraction technology with glass fiber filter membrane purification, which can improve the extraction effect of complex samples and samples with low RNA content. After the sample is treated with MagZol reagent and chloroform, the supernatant is added with ethanol to provide appropriate binding conditions, then transferred to the purification column and centrifuged. Macromolecular RNA can be efficiently bound to the membrane. Collect the filtrate containing small RNA, add more ethanol to adjust the binding capacity of small RNA, the pollutants can be efficiently washed away by second cleaning. Finally, the purified RNA was eluted by RNase free water.
Advantages
Kit Contents
| Contents | R431002 | R431003 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately. This Kit combines phenol/guanidine-based lysis of samples and silica membrane–based purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.