AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.
Detail
Description
AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.
Features
Sharp bands
Suitable for polyacrylamide gel electrophoresis
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 2,000 bp
Concentration
45.5 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months 4°C for 12 months -20°C for 36 months
Other Products
Small RNA Library Prep Kit for Illumina
Product Info
Document
Product Info
Overview
Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA
Simple and quick workflow: library could be prepared in less than 5 hours
No gel purification for selected types of samples
Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)
Complements Norgen’s Best-in-Class Total RNA (including microRNA) Purification Technology
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.
Storage Conditions and Product Stability Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.
Step
Component
Cat. 64600 (24 preps)
3′ AdaptorLigation to Template RNA
3′ Adaptor
30 µL
3′ Adaptor Ligation Master Mix
320 µL
T4 RNA Ligase 2 (Truncated)
35 µL
5′ Adaptor Ligation
5′ Adaptor
30 µL
5′ Adaptor Ligation Master Mix
320 µL
T4 RNA Ligase 1
35 µL
cDNA Synthesis from Ligated RNA Product
Reverse Primer
30 µL
cDNA Synthesis Master Mix
220 µL
TruScript ReverseTranscriptase
35 µL
PCR Amplification
2x NGS PCR Master Mix
1.32 µL
PCR Reverse Primer
81 µL
Forward Index Primer
Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610)
High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components:
Saliva Collection Funnel and Collection Tube
Collection Tube Cap
Norgen’s Saliva DNA Preservative contained within a sealed squeezable ampoule
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat. RU45400). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.
Saliva DNA Preservative
Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.
When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.
Kit Components
Cat. RU49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices
50
Donor Procedure Flowchart
1
Product Insert
1
Individual Saliva DNA Collection and Preservation Device Contents
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
NGS DNA Library Prep Kit Workflow
Kit features
Simple procedure: Reactions for all three steps are in one tube
Fast protocol
Total protocol time is around 1 hour
Hands-on time is only ~5 minutes
Guaranteed high library conversion efficiency as compared to other kits
Input DNA amount: from 50 ng to 1 ug
No insert concatemer ligation
Document
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
