Introduction

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details

Specifications

Features	Specifications
Main Functions	IVD5412 precast kit for kingfisher Flex
Applications	RT-PCR，PCR，NGS
Products	Viral DNA / RNA, body cell DNA / RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technolog
Process method	Manual or automatic
Sample type
Sample amount	200μl
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.

Advantages

Kit Contents

Cat.No	Reagent	IVD5412-F-96
PK/Carrier RNA		1x 24 mg/Bottle
Protease Dissolve Buffer Blue		1.8 ml/Bottle
Tip		1
Sample Plate (DW Plate)	500µl Buffer MLB/Well	1
Wash 1 Plate (DW Plate)	500µl Buffer MW1/Well	1
Wash 2 Plate (DW Plate)	500µl Buffer CW/Well	1
Beads Plate (DW Plate)	500µl CW & 20µl MPN/Well	1
Elute Plate (KF Plate)	90µl NFW/Well	1

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Introduction

The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent
Applications	qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method	Mini spin column
Purification technology	Silica technology, acidphenol / guanidine extraction technology (MagZol pretreatment technology)
Process method	Manual (centrifugation or vacuum)
Sample type	Blood, serum, plasma
Sample amount	250μl
Elution volume	≥20μl
Liquid carrying volume per column	800µl
Binding yield of column	100μg

Principle

0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.

Advantages

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – isolation of several samples can be completed in 40 minutes by using column purification method
• Safety – no phenol chloroform extraction required
• Sensitive – direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Contents	R416302	R416303
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	2 x 125
MagZol LS Reagent	60 ml	270 ml
Buffer RWC	20 ml	60 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.

Description

Fentanyl (FYL) Detection Kit (Rapid Lab) is a lateral flow chromatographic immunoassay for the qualitative detection of Fentanyl/Norfentanyl in liquid and powder substances at the cut-off concentration of 200 ng/mL.

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It is specific for Fentanyl screening with no significant cross reactivity to other opiates, such as Morphine and Heroin.

Interpretation of test results are: positive (one line), negative (two lines), invalid (no lines or no control line).

• • Sample: Liquid / Powder
• • Format: Cassette
• • Sensitivity: 200 ng/mL
• • Specificity: Fentanyl
• • Accuracy: > 98%
• • Time-to-Result: 10 minutes
• • Storage Condition: 2-30°C/36-86°F
• • Test Principle: Lateral Flow Immunoassay

For professional institutions use only, not for self-testing.

Learn More:

• Fentanyl Facts  from CDC
• Fentanyl Test Strips: A Harm Reduction Strategy

Rapid screening test for the qualitative detection of Fentanyl (FYL)
Format: 10 tests
Run Time: 10 Minutes