Overview

• Detection kits for Streptococcus uberis
• Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.

Streptococcus uberis TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Streptococcus uberis TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

Previous

Next

Click for expanded view

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM30850 (100 preps)	Cat. TM30810 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
S. uberis Primer & Probe Mix	280 μL	280 μL
S. uberis Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.

Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.

Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.

The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.

Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.

Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.

Methyltetrazine-PEG1-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Methyltetrazine-PEG1-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description 

The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Two reference bands — 75 kDa (red) and 25 kDa (green)

Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control 

Under suggested conditions, PM2600 ExcelBand™ 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage

The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Description 

The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. 

Range 

100 ~ 3,000 bp 

Concentration 

56 µg/ 500 µl 

Recommended loading volume 

5 µl/ well 

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months

The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. 

Overview

• Quantified standard to be used as a positive control or PCR quantification standard
• Vigorously quantified using multiple methods

Listeria monocytogenes has emerged as a significant foodborne pathogen that poses a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium. Due to its ability to survive high and low temperatures as well as low pH, it could resist various food processing technologies, as well as grow at food storage temperatures. L. monocytogenes is known to be associated with raw meat, unpasteurized milk and dairy products, vegetables, and seafood. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals being the most susceptible.

Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Listeria monocytogenes.
 

Details

Supporting Data

Figure 1 / 1

Previous

Next

Click for expanded view

Volume Provided	250 μL
DNA Quantity	2 x 104 copies per μL

Storage Conditions

Upon receipt, store Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard at -20^oC or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20^oC or lower.