Description
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
Features
- Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation.
- Fast and Easy– only few steps for preparation; suitable for time-saving transformation
- High efficiency– up to 109 cfu/μg
- Personalization– suitable for most E. coli strains
Kit Contents
| Component | Volume | |||||
| Champion™ CC Buffer | 20 ml | |||||
| SMO-Broth™ | 100 ml x 2 | |||||
| pUC19 Control Plasmid (10-4 μg/μl) | 5 µl | |||||
| Instruction Manual | 1 | |||||
| Champion™ Competent Cell Preparation Card | 1 |
Storage
4°C for 12 months
