[DL2000] ExcelDye™ 6X DNA Loading Dye, Green, 5 ml x 2
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The ExcelDye™ 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Detail
Description
The ExcelDye™ 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.15% Orange G
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Other Products
IL-6 / Interleukin-6 Control
Product Info
Document
Product Info
Name of Product
IL-6 / Interleukin-6 Control
Catalog Number
MQCO6 1
Short Info
This product is a Interleukin-6 Quality Control (two areas) for Milenia QuickLine IL-6 Test (MQL6 1).
This product is only available within the EU!
Method/Platform
control
Range/Assay Sensivity
two areas
Test Principle
The controls are designed only for internal Quality Control of Milenia QuickLine Il-6 Tests. Test results can only be evaluated with Milenia POCScan Reader.
Brief Instructions
Storage
2°C to 8°C
Components
IL-6 Control 1, IL-6 Control 2
Document
2 x 3 flasks This product is a Interleukin-6 Quality Control (two areas) for Milenia QuickLine IL-6 Test (MQL6 1).
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Extract viral RNA/DNA from non-cell/low cell content biological samples
Applications
RT-PCR，PCR，NGS
Products
Viral total nucleic acid
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount
200μl
Yield
2-10μg
Elution volume
≥30μl
Time per run
≤30 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
Advantages
Fast – several samples can be extracted in 20 minutes by column method
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid
Kit Contents
Contents
IVD4173
Purification Times
100 Preps
HiPure Viral Mini Column
100
2ml Collection Tubes
200
PK/Carrier RNA
50 mg
Protease Dissolve Buffer
5 ml
Buffer AL
30 ml
Buffer MW1*
44 ml
Buffer MW2*
50 ml
RNase Free Water
15 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Experiment Data
Document
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
NGS Library Quantification Standards With PCR Primers (Ion Torrent Platform)
Product Info
Document
Product Info
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.
The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.
NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.
Document
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
