【IT1000】EzRNA™ T7 High Yield RNA Synthesis Kit, 50 RXN
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The EzRNA™ T7 High Yield RNA Synthesis Kit is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Detail
Description
The EzRNA™ T7 High Yield RNA Synthesis Kit is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Features
High yield
Versatile- suitable for short and long transcripts
NTP premixed- Minimal pipetting and setup time
Compatible with CleanCap® Reagent AG
Lithium chloride included for RNA purification
Application
Generation of RNA from T7 promoter-driven DNA sequences
Suitable for subsequent cap-0 and cap-1 modification
Storage
-20°C for 12 months
Other Products
Propargyl-PEG8-NHS ester
Product Info
Document
Product Info
Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-DBCO is a homobifunctional PEG linker containing two DBCO groups and a hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG4-DBCO is a homobifunctional PEG linker containing two DBCO groups and a hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Brucella abortus is an intracellular, blood-borne parasite. It is a Gram-negative coccobacillus that causes an infectious and contagious disease called Brucellosis. The disease primarily affects cattle but it can also be transmitted to humans from infected animals and consuming their products. The disease can lead to great economic loss especially in the dairy and agricultural industry. The Brucella abortus genome contains two DNA chromosomes in a circular confirmation; the first chromosome is approximately 2.1 Mb and the second chromosome is approximately 1.2Mb. Unusually it does not contain any plasmids or genomic islands that relate to pathogenicity and lacks many other genes that code for common virulence factors including capsules, fimbriae, exotoxins, cytolysins, resistance forms, or antigenic variation. The most common mode of transmission to humans is through the ingestion of unpasteurized milk and cheese products as the bacteria are present in the milk glands of infected female cows. In cattle transmission can also be through ingestion but in addition, the bacteria can persist in the reproductive tracts of males, namely seminal vesicles, ampullae, testicles, and epididymides, allowing sexual transmission. In humans the bacteria enter macrophages by phagocytosis and then live in compartments of vacuolar space along the endoplasmic reticulum. They persist by inhibiting host apoptosis and go onto form chronic disease causing lesions in the liver, spleen, bone marrow and kidneys. In cattle the bacteria additionally infect the trophoblast epithelial cells, which provide nutrition to the embryo. The trophoblast cells eventually lyse, releasing further bacteria into the blood stream of the embryo. The B. abortus cells in the blood stream go on to colonize the placenta and fetus in pregnant female cows, resulting in abortion of the fetus. Abortion can also result from insufficient anti-Brucella activity in the amniotic fluid. In humans, the disease can be either acute or chronic and some of the symptoms include fluctuating fever, chills, sweating, headache, muscle pain and weight loss. Once a person becomes infected they are prescribed a combination of tetracycline and streptomycin for 3-6 weeks. In cattle, additional symptoms include arthritic joints and retained after-birth.
Document
Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
