【IT1100】EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP), 50 RXN
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The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Detail
Description
The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Features
High yield
Versatile- suitable for short and long transcripts
NTP premixed- Minimal pipetting and setup time
Compatible with CleanCap® Reagent AG
Lithium chloride included for RNA purification
Application
Generation of RNA from T7 promoter-driven DNA sequences
Suitable for subsequent cap-0 and cap-1 modification
Storage
-20°C for 12 months
Other Products
BP MagPure A4 XP
Product Info
Document
Product Info
Introduction
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Details
Specifications
Features
Specifications
Main Functions
Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP)
Applications
NGS, DNA library
Purification technology
Magnetic beads technology
Process method
Manual (centrifugation or vacuum)
Sample type
DNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount
Appropriate
Recovery
80%
Operation time
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
High recovery – up to 80%
High throughput – using magnetic beads purification technology
Kit Contents
Contents
BP-5
BP-50
BP-500
MagPure A4 XP
5 ml
50 ml
500 ml
Storage and Stability
MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
Document
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
Features
High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
Document
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.
