[RQ2110] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX), 200 RXN

Overview

• Clean-up & concentrate total RNA (including miRNA) in minutes
• Clean-up & concentrate RNA from TRIzol^®, TRI Reagent^®, etc
• Clean-up RNA from contaminants including enzymes, primers, nucleotides
• Rapid spin-column protocol, elute in 20 µL
• 96-well format for high throughput processing & Micro-Elute spin column formats for 8 µL are available
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol^® and TRI Reagent^®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

RNA Clean-Up and Concentration Kit (Spin Column)

Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.

RNA Clean-Up and Concentration 96-Well Kit (High Throughput)

Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.

RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute)

Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.

Protocol

Details

Supporting Data

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Kit Specifications (Spin Column)
Maximum Column Binding Capacity	35 μg
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material	35 μg of RNA
Minimum Elution Volume	20 μL
Time to Complete 10 Purifications	20 minutes
Average Recovery	≥ 90%

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 23600 (50 preps)	Cat. 43200 (100 preps)	Cat. 25100 (192 preps)	Cat. 61000 (50 preps)
Buffer RL	40 mL	2 x 40 mL	2 x 40 mL	40 mL
Wash Solution A	38 mL	2 x 38 mL	2 x 38 mL	38 mL
Elution Solution A	6 mL	2 x 6 mL	2 x 20 mL	6 mL
Column Activation Solution	–	–	–	30 mL
Micro Spin Columns	50	100	–	–
Micro-Elute RNA Spin Columns	–	–	–	50
96-Well Plate	–	–	2	–
Adhesive Tape	–	–	4	–
Collection Tubes	50	100	–	50
96-Well Collection Plate	–	–	2	–
Elution Tubes (1.7 mL)	50	100	–	50
96-Well Elution Plate	–	–	2	–
Product Insert	1	1	1	1

• R-RPE is manufactured from a red algae
• Protein based fluorescent particles specifically desinged for lateral flow applications
• Highly absorptive fluorescent molecule with excellent detectability

Description

R-Phycoerthyrin (R-RPE) Streptavidin Conjugate designed for Lateral Flow

Our Streptavidin R-Phycoerythrin conjugates are manufactured from a red algae and the first of its kind protein based fluorescent particles specifically desinged for lateral flow applications. R-RPE is a highly absorptive fluorescent molecule that has excellent detectability. It is the fluorochrome of choice when the brightest signal is needed and is therefore used most often when high sensitivity is essential for detectability and/or accuracy.

Consistent lot-to-lot performance resulting from continuous culture of source organisms and high purity.
– Very high water solubility
– Homogeneous structure with defined molecular weights
– Total control on growth conditions and nutrition, which avoids contamination from extraneous organisms and waste found in the open ocean. Proteins are harvested at the optimal stage of the growth cycle to assure uniform product characteristics. The pigment is extracted and stabilized within minutes of harvest, virtually eliminating risks from the action of proteases.

250ul stock ready to use for lateral flow

50mL lateral flow running buffer designed for Streptavidin R-Phycoerythrin particles 

• Excitation max: 566nm

• Emission max: 575nm

R-RPE is manufactured from a red algae

Protein based fluorescent particles specifically desinged for lateral flow applications

Highly absorptive fluorescent molecule with excellent detectability

Overview

• Extract total RNA, including virus & viroid RNA
• Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
• Isolate total RNA (including microRNA) without phenol
• Isolated RNA is of high quality, integrity and diversity
• Also available in 96-well format for high throughput applications
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. 

The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.  

Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications.  Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

Figure 1 / 4

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Kit Specifications – Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi	50 mg 1 x 106 cells 50 mg (wet weight)
Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves	60 μg 60 μg 32 μg 35 μg 30 μg
Time to Complete 10 Purifications	30 minutes

* Yield will vary depending on the type of sample processed.

* Yield will vary depending on the type of sample processed.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits

Plants

Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves

Fungi

Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima

Component	Cat. 25800 (50 preps)	Cat. 31350 (100 preps)	Cat. 25850 (250 preps)	Cat. 31900 (192 preps)
Lysis Buffer C	60 mL	1 x 30 mL, 1 x 60 mL	3 x 60 mL	2 x 60 mL
Wash Solution A	38 mL	38 mL	1 x 18 mL, 2 x 38 mL	2 x 38 mL
Elution Solution A	6 mL	6 mL	20 mL	20 mL
Filter Columns	50	100	250	–
Spin Columns	50	100	250	–
96-Well Plate	–	–	–	2
Adhesive Tape	–	–	–	4
Collection Tubes	100	200	500	–
96-Well Collection Plate	–	–	–	2
Elution Tubes (1.7 mL)	50	100	250	–
96-Well Elution Plate	–	–	–	2
Product Insert	1	1	1	1