The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.
Detail
Description
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.
Features
Reverse transcription at wide temperature range (42-60°C)
High specificity
Suitable for fast cycle program
With ROX reference dye
Storage
Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Features
High Stability
Fast Hot Start
High Sensitivity
Low Background
Suitable for Fast Program
Smart Blue Contrast Dye
With ROX Reference Dye
Storage
Protect from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
Document
The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) also includes ROX reference dye for normalizing the fluorescent reporter signal in real-time quantitative PCR. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
Document
For the detection of the SARS-CoV-2 variants with the N501Y mutation (UK, South Africa and Brazil) Rapid detection of specific detection profiles High priming efficiency Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
GL21MC high speed refrigerated centrifuge Application:
Widely used in the area of animal and plant molecular biology, cell biology, clinical applications, bio-engineering, mass preparation and blood, protein precipitation, Separating the biology, viruses, sub-cell, cell and the chemicals in the cell.
Features:
1.Brushless frequency motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration. 3.Import compressors fluorine free, double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.. 4. Microprocessor control system, the 10 inches LCD display indicates all the parameters in running, 5. There are more than 20 kinds of rotors for your choice, especial the continuous rotors which help you purify a large number of test solution continuously. 6. There are 30 kinds of program and 10 kinds of accelerating and decelerating speed for your choice. 7.ω2t centrifugation is allowed, which make you have the ideal centrifugation result. 8. 20 kinds of rotors for your choice, especial the continuous rotor help you purify a large amount of test solution continuously. 9. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. 10. The centrifuge body is made of high quality steel, safe and reliable. 11. 3 tiers protection steel cover.
Technical parameters:
Max speed
21000r/min
Max RCF
46140*g
Time range
1~99H59min
Speed accuracy
±50r/min
capacity
6*500ml
Noise
≦65dBA
Weight
235KG
Dimension
865*728*1240mm
Packaged weight
285KG
Packaged dimension
1000*850*1510mm
Temperature range
-20~40℃
Temperature accuracy
±1℃
Power
AC110/220V, 50-60HZ,35A
Rotor identification
Automatic identification
Package
wooden box
Matched Rotors for GL21MC:
Order No.
Rotor
Max speed (r/min)
Max capacity (ml)
Max RCF (*g)
30108
Fixed rotor
21000
12*10ml
44440
30117
Fixed rotor
20000
16*10ml
46140
30111
Fixed rotor
20000
6*50ml
44700
30118
Fixed rotor
20000
8*30/20ml
42040
30119
Fixed rotor
15000
8*50ml
27540
30112
Fixed rotor
15000
6*70ml
25480
30120
Fixed rotor
12000
8*100ml
19610
30121
Fixed rotor
11000
4*300ml
18120
30122
Fixed rotor
10000
6*300ml
15730
30123
Fixed rotor
10000
6*500ml
18340
30128
Continuous centrifugal rotor
17000
1000ml
31390
30129
Continuous centrifugal rotor
14000
1800ml
21710
30130
Continuous centrifugal rotor
8000
3000ml
9450
30131
Continuous centrifugal rotor
8000
1800ml
8600
30132
Interval rotor
17000
1000ml
31390
30133
Interval rotor
12000
1200ml
19350
30134
Interval rotor
10000
3000ml
14890
30135
Vertical rotor
20000
16*5ml
38340
Vertical rotor
18000
8*30ml
32650
30180
Swing rotor
15000
4*5ml
21670
30181
Swing rotor
13000
4*30ml
23580
30182
Swing rotor
10000
4*50ml
16240
30694
Bucket rotor
4000
4*4*96holes
2840
30696
Swing rotor
4000
4*500ml
3380
Document
GL21M max. capacity can hold 800ml bottles 4pcs, max speed over 20000rpm Model: GL21M
