Description
The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.
Features
- 5’→3’ DNA polymerase activity
- 3’→5’ exonuclease (proofreading) activity
- Suitable for GC-rich templates
- High reaction rate: 7 seconds/kb
- High fidelity: 70 times higher than Taq polymerase
- Generates blunt end amplicons
- Vast elongation capability (up to 40 kb)
- Thermo-stable for more than 10 hrs at 95°C
Storage
[TF3000] G-HiFi™ DNA Polymerase
-20°C for 24 months
