[TK1000] ExcelTaq™ Klen-Taq DNA Polymerase, 5 U/μl, 500 U

Overview

• High yield and high purity DNA ready for any application
• Available in a variety of formats to properly suit your needs
• Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1

A custom designed PACE® Genotyping Assay, designed to your target sequence. Compatible with all PACE genotyping master mixes.

About

PACE (PCR Allelic Competitive Extension) genotyping chemistry is a homogeneous, PCR-based allele-specific technology for the analysis of DNA sequence variants, most commonly SNPs (Single Nucleotide Polymorphisms) and Indels (insertion / deletions).

PACE genotyping chemistry is comprised of two parts:

1. PACE Genotyping Assay: two allele-specific forward primers and one common, reverse primer. The allele-specific forward primers each have different 3′ terminal bases reflecting the target variant, and a unique 5’ tail sequence which is incorporated as part of the fluorescent signal mechanism.
2. PACE Genotyping Master Mix: containing all remaining components required for PCR and the generation of fluorescent signals. PACE Genotyping Master Mix contains a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals (FAM and HEX) corresponding to the assay genotypes.

When combined with sample DNA, these components create a PACE Genotyping Reaction, as illustrated in the figure below.

We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. PACE Genotyping Assays are available to purchase either Validated and Unvalidated. Validated assays require customer DNA to validate and optimise, for guaranteed performance. Unvalidated assays are designed in silico and supplied untested.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• Template DNA
• PCR-grade water
• PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

STEPS TO YOUR PACE GENOTYPING ASSAY DESIGN

1. Place your order on this page. Our support team will contact you by email.
2. Fill out an Assay Design Template form with all the information we need to process your custom PACE Genotyping Assay order. We will email you a copy of the template when we first contact you, or your can download a copy here.
3. Email your completed Assay Design Template to support@3crbio.com
4. Using the information you provide us, we will create your PACE Genotyping Assay designs, order the oligos, and send you design sequences.
5. Once we receive the oligos, we assemble the assay(s) and then ship an aliquot to you (unvalidated) or test on your DNA samples before shipping the aliquot to you (validated).

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

Template DNA

PCR-grade water

PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

The Flex caddy allows the Heater-Shaker GEN1 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Heater-Shaker. Caddies and calibration adapters are specific to the type of module.

Note: This item does not include the Heater-Shaker. The module is available with or without the Flex Caddy and Calibration tool: Heater-Shaker Module GEN1

The Flex caddy allows the Heater-Shaker GEN1 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Heater-Shaker. Caddies and calibration adapters are specific to the type of module.

Note: This item does not include the Heater-Shaker. The module is available with or without the Flex Caddy and Calibration tool: Heater-Shaker Module GEN1