The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Detail
Description
The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Features
5’→3′ DNA polymerase activity
No detectable 3’→5′ exonuclease (proofreading) activity
Generates PCR products with 3′-dA overhangs
High throughput PCR
High yield PCR
High reproducibility, with reduced pipetting errors
Compatible with most anticoagulants
Applications
Direct amplification of DNA from blood samples
High throughput screening without DNA purification
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.2-0.6ml
Elution volume
≥30μl
Time per run
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
IVD5432
Purification Times
200
MagPure Particles G
14 ml
Carrier RNA
310 μg
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer MLK
250 ml
Buffer MAW1
250 ml
Buffer MW2*
2 x 50 ml
Elution Buffer
60 ml
Contents
IVD5432-F-96A
IVD5432-F-96B
IVD5432-F-96C
Sample amount
200～350μl
400~700μl
Carrier RNA
110 μg
110 μg
Proteinase K
50 mg
100 mg
Protease Dissolve Buffer
5 ml
6 ml
Elution Buffer
15 ml
15 ml
Tip
1
1
Sample Plate A
500μl Buffer MLK
500μl Buffer MLK
Sample Plate B
/
500μl Buffer MLK
Wash Plate 1
700μl Buffer MAW1
700μl Buffer MAW1
Wash Plate 2
25μl Buffer MPG2700μl Buffer MW2
25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3
700μl Buffer MW2
700μl Buffer MW2
Elution Plate
/
/
Contents
IVD5432-TL-06A
IVD5432-TL-06B
IVD5432-TL-06C
Sample amount
300~350μl
600~700μl
900~1050μL
Carrier RNA
310 μg
310 μg
310 μg
Proteinase K
50 mg
100 mg
150 mg
Protease Dissolve Buffer
6 ml
6 ml
10 ml
Elution Buffer
15 ml
15 ml
15 ml
DA-Tip
12
12
12
Row 1/7
600μl Buffer MLK
600μl Buffer MLK
600μl Buffer MLK
Row 2/8
/
600μl Buffer MLK
600μl Buffer MLK
Row 3/9
600μl Buffer MAW1
600μl Buffer MAW1
600μl Buffer MLK
Row 4/10
20μl Buffer MPG2600μl Buffer MW2
20μl Buffer MPG2600μl Buffer MW2
30μl Buffer MPG2900μl Buffer MAW1
Row 5/11
600μl Buffer MW2
600μl Buffer MW2
900μl Buffer MW2
Row 6/12
/
/
/
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Document
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Ammonia, Nitrogen, YAN
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
0.2 to 7 µg of ammonia per assay
Limit of Detection:
0.07 mg/L
Reaction Time (min):
~ 5 min
Application examples:
Grape juice, wine, fruit juices, soft drinks, dairy products (e.g. milk), dietetic food, soy sauce, eggs and egg products, cheese, meat, processed meat, seafood, bakery products (and baking agents), fertilisers, pharmaceuticals, tobacco, cosmetics, water, Kjeldahl analysis, paper (and cardboard), water and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by MEBAK
Ammonia Assay Kit, for the rapid measurement and analysis of ammonia in all samples, including grape juice and wine (and other foods/beverages).
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
