Introduction

This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue using economic salt out method
Applications	PCR, enzyme digestion, Southern hybridization,etc.
Purification technology	Salting out method
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissue
Sample amount	Unlimited
Elution volume	≥100μl
Time per run	Variation with sample amount

Principles

Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.

Advantages

• Safe – without phenol chloroform extraction
• Environment friendly – the reagents used are safe, non-toxic and without pollution
• High molecular weight – the molecular weight of genomic DNA is about 50-150kb
• High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
• Unlimited sample size – solution type operation, sample volume can be adjusted at will
• Cost performance – the most economical nucleic acid extraction technology

Kit Contents

Contents	D331201	D331202	D331203
Purification Times	1 g	5 g	50 g
Proteinase K	330 µl	1.8 ml	18 ml
Buffer WTL	33 ml	160 ml	2 x 800 ml
Buffer PPS	12 ml	55 ml	500 ml
RNase Solution	330 µl	1.8 ml	18 ml
Buffer TE	12 ml	60 ml	200 ml

Storage and Stability

RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.

Overview

• Rapid and simple procedure to generate digested peptides
• Simultaneous digestion, purification and concentration at once
• Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry
• Peptides are ready for applications such as mass spectrometry and SDS-PAGE
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format.  Trypsin is added to the protein sample and bound to the column.  Salts are washed away and the trypsin is then activated to digest proteins.  Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry.  Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation).   The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.

Details

Supporting Data

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Kit Specifications
Maximum Protein Input	15 μg
Minimum Protein Input	2 μg
Minimum Elution Volume	30 μL
Time to Process 10 Purifications	20 minutes (Plus a 1-hour incubation)

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17500 (25 preps)
Wash Solution C	30 mL
Binding Buffer A	4 mL
Column Activation Buffer	3 mL
Micro Spin Columns	25
Collection Tubes	25
Elution tubes (1.7 mL)	25
Product Insert	1

* Optical system: equipped with infinite flat field optical system, the whole machine mildew prevention design and large field eyepiece, with beautiful appearance, clear imaging, broad vision, convenient operation and other characteristics, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units

* Observation head device: the cylinder can rotate 360 degrees, the displacement of the center of the mirror is 0.06mm; 30 tilt; the hinge type binocular observation (the magnification difference between left and right systems is 2.0%; the end position difference is 0.1mm at zero vision) and the third party connection port, the pupil distance adjustment range is 50-75mm, the fixed observation cylinder head is internal hexagon screw or select portable screw.

* Eyepiece configuration: random eyepiece WF10X / 22mm; the magnification accuracy of eyepiece shall not exceed ± 2%. There is a card slot on the eyepiece tube to lock the eyepiece to prevent falling. Binocular vision is adjustable, making binocular observation easier;

* Objective: Broadband coated ED-N195 pure copper infinite remote flat field achromatic objective; objective magnification accuracy not exceeding ± 5%; 4X, 10X, 40X (spring), 100X (spring, oil); objective error 10-40 Aim 0.06mm; 40-100 Aim 0.03mm

* Loading platform: triangular rail XY composite mechanical moving loading platform; 0.005 lateral to 5N horizontal force, corrosion resistance and wear resistance. Rectangular, size 190X140mm, stroke 6090mm, minimum read value 0.1 mm.

Parameter of microscope

Specification configuration	TL36A	Binocular observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm
	TL36B	Three-eye observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm.
	TL36M	Three-eye viewing head with 6.3 megapixel imaging system CMOS camera
	TL36P	Three-eye viewing head, equipped with 10.1 inch integrated flat panel display
Eyepiece	Wide-angle eyepiece WF10X,field of view 22mm,optional WF16X,WF25X
Converter	Quadruple Revolving converter
0bjective	Flat field achromatic objective at infinite distance,4X,10X,40X,100X(oil)
Condenser	ABBE N.A.1.25Condenser with Iris Diaphragm &Filter
Stage	Double Layer Mechanical Stage,  Size1190X140mm,              Moving Range : 60mmx90mm
Focusing	0.02mmCrude motion, micromotion and coaxial focal adjustment system with a minimum focal range of 0.02mm Coarse and Fine Focusing
Light source	3W LED,with Rechargeable Battery,Type C USB cable
Attachments	Dust bag,Type C power cord

TL 36 series biological microscopes are equipped with infinite distance flat field achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units