Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
Detail
Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications
qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (vacuum)
Sample type
Serum, plasma and other cell-free fluid samples
Sample amount
1-5ml
Elution volume
≥50μl
Time per run
≤60 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.
Advantages
High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration – low elution volume, ensuring high nucleic acid concentration
High purity – low alcohol binding method, completely removing inhibitor and protein pollution
High recovery – DNA can be recovered at thelevel of PG by silica gel column purification
Kit Contents
Contents
IVD3182
Purification Times
50
Buffer ACL
250 ml
Buffer ACB*
300 ml
Buffer DCW1*
22 ml
Buffer DCW2*
10 ml
Proteinase K
540 mg
Protease Dissolve Buffer
30 ml
Carrier RNA
110 μg
Nuclease Free Water
20 ml
HiPure CFDNA Mini Columns
50
2 ml Collection Tubes
100
Extender Tube
50
Vac-Connector
50
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
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Product Info
Document
Product Info
Product Description
Product Description:
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Amp-future bio, a leading Chinese manufacturer of detection kits, is proud to introduce its new Pathogen Detection Kit. This innovative swab test kit is specially designed for rapid detection of influenza and other infectious diseases, delivering results in as little as 5-20 minutes. Featuring a storage temperature of -20ºC, this test kit is highly reliable and easy to use. It is ideal for hospitals, medical centers, and other healthcare facilities to quickly and accurately detect pathogens. As a trusted name in the industry, Amp-future bio guarantees the highest quality and safety when it comes to its Pathogen Detection Kit, making it the perfect choice for all your rapid test kits and infectious disease test kits needs.
Support and Services:
Pathogen Detection Kit technical support and services are offered to customers to ensure optimal performance and usability of the product. Technical support is available through our customer service hotline as well as our online customer portal. Our technical support team is available to answer any questions and provide guidance for product installation, troubleshooting, and maintenance. Additionally, our team provides software updates and upgrades, as well as access to our library of technical documents and resources. We also offer training and education services for customers who wish to better understand the product and its capabilities.
Packing and Shipping:
To ensure that our Pathogen Detection Kit arrives to you in perfect condition, we take special care in packaging and shipping.
Before shipping, all kits are carefully inspected for product quality. They are then bubble-wrapped and sealed in a box to provide the highest level of protection.
We use a reputable shipping company with tracking options and signature confirmation. Depending on the shipping destination, the package may require additional documentation, such as a Certificate of Origin or an invoice.
We strive to deliver your order as quickly and cost-effectively as possible. We offer same day shipping for orders placed before noon EST.
FAQ:Q1: What is Amp-future bio Pathogen Detection Kit?A1: Amp-future bio Pathogen Detection Kit is a high-performance product developed by China for rapid detection of pathogenic microorganisms.Q2: What microorganisms can be detected by this kit?A2: The Amp-future bio Pathogen Detection Kit can detect a wide range of pathogenic microorganisms, including bacteria, viruses, and parasites.Q3:Is the kit easy to use?A3: Yes. The Amp-future bio Pathogen Detection Kit is designed for easy operation and use. It comes with detailed instructions for use.Q4: Is the kit safe?A4: All components of the Amp-future bio Pathogen Detection Kit are strictly tested to ensure safety. The kit is safe and reliable to use.
Document
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells,
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
Document
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.
Document
Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.
