This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
Detail
Introduction
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
Details
Kit Contents
Contents
D312402
D312403
Purification Times
50
250
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer BGL
40 ml
180 ml
Buffer GXP
30 ml
150 ml
Buffer GW1*
13 ml
53 ml
Buffer GW2*
20 ml
2 x 50 ml
DTT Powder
235 mg
2 x 235 mg
Proteinase K
48 mg
240 mg
Protease Dissolve Buffer
5 ml
15 ml
Elution Buffer
15 ml
60 ml
Storage and Stability
This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.
Other Products
N-(Acid-PEG4)-N-bis(PEG4-Propargyl)
Product Info
Document
Product Info
N-(Acid-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the acid can be reacted with amines or alcohols to form stable amides or esters.
Document
N-(Acid-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the acid can be reacted with amines or alcohols to form stable amides or esters.
Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile urine input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into flexible elution volumes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Background
Recent evidence indicates that cell-free circulating RNA (cf-RNA) including exosomal RNA in urine contains valuable information for the discovery of biomarkers that can help for the early detection of certain cancer types and for monitoring the disease status as well as for the detection of any infectious pathogens. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acid for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum but with a lower concentration; (3) Nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).
Urine Cell-Free Circulating RNA Purification Mini Kit
For sample volumes ranging from 2 mL to 10 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL
All sizes including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen
*Please check page 6 of the product insert for average yields and common RNA quantification methods.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
For enrichment of bacteria of cosmetics and disposable hygiene products.
Principle:
Casein peptone and soybean peptone provide the nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; dipotassium hydrogenphosphate as a buffer; lecithin and Tween 80 can neutralize preservatives and can play a dispersed material in the emulsion features, lecithin can neutralize quaternary ammonium salt, Tween 80 neutralizes phenols, hexachlorophene, formalin, a combination that can neutralize and ethanol.
Formulation(per liter):
Casein peptone 17g
Soy peptone 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose 2.5g
Lecithin 1g
Tween 80 7g
Final pH7.2 ± 0.2
How to use:
1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, distribute into flask,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
