D5001 MagPure Gel Pure DNA Kit

Introduction

A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
Applications	PCR, NGS, chip analysis, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Agarose Gel, PCR products, enzymatic reaction solution
Sample amount	Agarose Gel: <0.3g, PCR products: ≤50µl
Recovery	80%
Elution volume	≥30μl
Time per run	≤30 minutes

The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.

Advantages

High recovery efficiency – up to 80% DNA recovery
General – recover DNA from gel or enzyme-driven reaction solutions such as PCR

Kit Contents

Contents	D500101	D500102	D500103
Purification Times	50 Preps	500 Preps	5000 Preps
Buffer GDP	30 ml	250 ml	3 x 900 ml
MagPure Particles	1.6 ml	15 ml	3 x 50 ml
Buffer DW1	20 ml	180 ml	2 x 800 ml
Elution Buffer(10mmTris, pH8.5)	10 ml	60 ml	500 ml

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data