Introduction
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell, whole blood |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount | Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture |
Kit Contents
| Contents | IVD3020B-96 | IVD3020B |
| Purification Times | 96 Preps | 200 Preps |
| MagPure Particles N | 2.5 ml | 5 ml |
| DNase I | 2 x 600 µl | 4 x 600 µl |
| DNase Buffer C | 60 ml | 120 ml |
| Buffer RLC | 60 ml | 120 ml |
| Buffer MCB* | 60 ml | 150 ml |
| Buffer GW1* | 44 ml | 110 ml |
| Buffer MW2* | 50 ml | 100 ml |
| RNase Free Water | 20 ml | 60 ml |
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.
