Introduction
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
| Features | Specifications |
| Main Functions | Extract viral DNA/RNA from 200μl cell-free samples by magnetic beads |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant |
| Sample amount | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
- Fast – several samples can be extracted in 40 minutes by column method
- High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
| Contents | IVD5412 |
| Purification Times | 200 Preps |
| MagPure Particles N | 5 ml |
| PK/Carrier RNA | 50 mg |
| Protease Dissolve Buffer Blue | 5 ml |
| Buffer MLB* | 120 ml |
| Buffer MW1* | 53 ml |
| RNase Free Water | 30 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
