C13200 Collection Tubes

Description 

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg²⁺ concentration as compared to other DNA polymerase products. 

Features

• 5’→3′ DNA polymerase activity
• 3’→5′ exonuclease (proofreading) activity 
• High reaction rate (up to 1 kb/10 seconds)
• High fidelity, 70 times higher than Taq DNA polymerase 
• Blunt end amplicons
• Thermo-stable: half-life is more than 10 hrs at 95°C 

Storage

[TF1000] SMO-HiFi™ DNA Polymerase

-20°C for 24 months

The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.

Name of Product

Schistosoma mansoni – IgG ELISA

Catalog Number

AF 9600

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample will bind to Schistosoma mansoni antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.

Brief Instructions

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, enzyme buffer, stopping solution

12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Schistosoma mansoni and Schistosoma haematobium in human serum.

Introduction

Intended Use

For the enumeration of enterococci in water and other liquids by the membrane filtration technique.

Principle and Interpretation

The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.

Formulation

Ingredients	/liter
Tryptose	20.0g
Yeast extract	5.0g
Glucose	2.0g
Disodium hydrogen phosphate	4.0g
Sodium azide	0.4g
2,3,5-Triphenyl Tetrazolium chloride	0.1g
Agar	10.0g
pH 7.2±0.1 at 25°C

Preparation

Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.

Quality Control

Cultural characteristics observed after incubation at 34-38°C for 40-48hours

Quality control strains	Growth	Colony color
Enterococcus faecalis ATCC29212	Good growth,PR≥0.5	deep red coloured colonies
Escherichia coli ATCC25922	Total inhibition	–

Sorage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……