Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
Detail
About
PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
Other Products
HCM131 Sorbitol MacConkey Agar Base
Product Info
Document
Product Info
Introduction
Intended Use
For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials
Principle and Interpretation
Enzymatic digest of casein and enzymatic digest of animal tissue provides nitrogen source, vitamins and growth factors; sodium chloride maintains balanced osmoticpressure; sorbitol is a fermentable sugar; No. 3 bile salt and crystal violet inhibit Gram-positive bacteria, potassiumtellurite inhibits non-o157 Escherichia coli, and cefixime inhibits Proteus; neutral red is a pH indicator; and agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Enzymatic digest of casein
17.0g
Enzymatic digest of animal tissue
3.0g
Sorbitol
10.0g
Bile salt No.3
1.5g
Sodium chloride
5.0g
Neutral red
0.03g
Crystal violet
0.001g
Agar
15.0g
pH 7.1±0.2 at 25°C
Preparation
Weigh 51.5g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, dispense into Erlenmeyer flasks, and sterilize at 121℃ for 15min. Melt the sterilized culture medium and cool it to about 45℃, add 1 tube of supporting reagent (SR0240) (0.25mg potassium tellurite and 0.005mg Cefixime) to every 100mL of basal culture medium, mix and pour into the plate.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 18-24hours
Quality control strains
Growth
Colony color
Escherichia coli ATCC25922
PR≥0.7
pale pink to red
Escherichia coli 0157:H7 NCTC12900
PR≥0.7
colorless
Staphylococcus aureus ATCC 25923
Total inhibition
–
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials Principle and Interpretation Enzymatic ……
Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.
About
Step into the future of high-throughput PCR genotyping with PACE Nano! Backed by decades of expertise in high-throughput genotyping reagent manufacturing, our groundbreaking product is engineered specifically to excel in low and ultra-low volume reactions. Say goodbye to performance variability as PACE Nano Genotyping Master Mix and PACE Genotyping Assays raise the bar for reliability and performance in the field.
With superior cost-effectiveness and versatile compatibility, PACE Nano Genotyping Master Mix ensures consistent results across diverse platforms, samples, and markers, including 384- and 1536-well PCR, Array Tape™, SNPline™ and Gene Mathematica from HC Scientific, available from 3CR Bioscience, supporting reaction volumes as low as 0.8 µL. Join the revolution today and elevate your research with PACE Nano.
Cost-Effective: Produce more high-quality data faster and at a much lower cost compared to competitors, reducing reagent expenses without compromising on quality.
Optimised for Low and Ultra-Low Volume PCR Genotyping: PACE Nano Genotyping Master Mix is specifically tailored for enhanced performance in miniaturised PCR genotyping, for reaction volumes as low as 0.8 µL and for high-throughput and automated processing with accurate, reliable results.
Consistent Data Across Assays: PACE Nano reduces variability in assay performance, enhancing the robustness and efficiency of high throughput, miniaturised and automated workflows.
Backed by 20 Years of Expertise: Designed and manufactured with 20 years of experience in high-throughput genotyping reagents, ensuring reliability and quality.
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REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
Document
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
