Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
Detail
About
PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Kit contents
Champion™ Competent Cells
pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
Champion™ Transformation Protocol Card
Shipping condition
Throughout the shipping process, the temperature is maintained under -70°C.
Storage and expiration
Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.
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Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.
Heat sealing is a quick and cost effective method of plate sealing
Our Gas PermASeal II Heat Seal is a paper with a grid lacquer coating which is porous and gas permeable
The Gas PermeASeal II Heat Seal is compatible with polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
The seal can be removed by peeling, or it can be pierced with a pipette tip manually or using a liquid handling robot
The Seal has a temperature range from -20°C to 80°C
It can be used for insect and seed storage, as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in roll format compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
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Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.
IST-126 QuickSeal DMSO X FilmTM Self Adhesive Sealing Film
Product Info
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Product Info
Overview
Strong adhesive seal with cross-cut windows. This seal is peelable and suitable for 96 well plates (auto samplers, HPLC, sequencers).
This strong adhesive seal was developed to facilitate easy sample removal with a manual or automated system
The seal is optically clear, being made from a transparent polyester film, and has a strong adhesive applied across the underside of the seal, with 96 round windows which align to the 96 wells of a plate
The optical windows are cross-cut, allowing for easy access to the sample wells with a tip or probe with minimal pressure
For all adhesive seals, the best sealing results are achieved using our Hand Roller
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Strong adhesive seal with cross-cut windows. This seal is peelable and suitable for 96 well plates (auto samplers, HPLC, sequencers).
