Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
Detail
Cell Culture Flask T25
Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
Other Products
[DM2160] FluoroBand™ 100 bp Fluorescent DNA Ladder, 500 μl
Product Info
Document
Product Info
Description
The DM2160 FluoroBand™ 100 bp Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2160 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 1,500 bp
Concentration
52.2 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months 4°C for 12 months -20°C for 24 months
Document
The DM2160 FluoroBand™ 100 bp Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2160 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results indicate that HL-dsDNase has minimal impact on RNA quality and quantity.
HL-dsDNase is an engineered version of dsDNase that is rapidly and completely inactivated by incubation for 5 minutes at 58°C with 1 mM DTT at pH 8.0 or above. Chemical inactivation in downstream compatible RT or PCR buffers allows milder heat inactivation and, in some cases, skipping heat inactivation altogether. This makes HL-dsDNase very useful for removal of DNA from RNA preparations since the enzyme may be inactivated using various strategies with reduced risk of auto-degradation of RNA in the presence of magnesium, making HL-dsDNase an ideal enzyme when working with small volumens of RNA.
HL-dsDNase is also an excellent choice for removal of unwanted external DNA from samples prior to analysis of nucleic acids protected by biological membranes, e.g., bacteria, viruses, and sperm. Especially if using downstream analysis methods that might be affected by host cell DNA, as metagenomic sequencing and STR-profiling. The easy inactivation of HL-dsDNase makes it a fast and efficient alternative to methods as differential extraction, where sample material is often lost.
Easily heat-inactivated by very moderate heat treatment
High specific activity
Useful for removal of DNA from RNA preps
Properties
Document
HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results indicate that HL-dsDNase has minimal impact on RNA quality and quantity.
Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.
2X PCR Master Mix (1 Vial, 100 Reactions) – Sufficient reagent for 100 x 20 µL reactions
Storage Conditions and Product Stability Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.
