Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
Detail
Cell Culture Flask T175
Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
Gamma radiation sterilization
Non Byrogenic Nase/Bnase free
Other Products
96-Well Universal Bar Magnetic Plate
Product Info
Document
Product Info
Permagen’s most universal bar magnet plate. From PCR plates to almost any microplate including deep well, flat bottom, pyramid bottom, round bottom, full-skirt PCR, and 1/2 skirt PCR the MSPU650 has you covered
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic bead
Permagen’s most universal bar magnet plate. From PCR plates to almost any microplate including deep well, flat bottom, pyramid bottom, round bottom, full-skirt PCR, and 1/2 skirt PCR the MSPU650 has you covered
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.
Details
Specifications
Features
Specifications
Main Functions
Extract viral RNA from 140μl cell-free samples
Applications
RT-PCR, Northern hybridization, and various virus detection
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cell-free body fluid or culture medium
Sample amount
140μl
Elution volume
≥15μ
Time per run
≤25 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
The sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.
Advantages
Fast – several samples can be extracted in 20 minutes by column method
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid
Kit Contents
Contents
R417102
R417103
Purification Times
50 Preps
250 Preps
HiPure Viral Micro Columns
50
250
2ml Collection Tubes
100
500
Buffer VRL
50 ml
200 ml
Carrier RNA
310 µg
3 x 310 µg
Buffer VHB*
13 ml
110 ml
Buffer RW2*
20 ml
2 x 50 ml
Nuclease Free Water
10 ml
30 ml
Storage and Stability
Carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions
Document
Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.
