96S Super Magnet Plate

Overview

• Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
• Isolate full diversity of RNA from large RNA down to small and microRNAs
• Eliminates PCR inhibitors including humic acids
• High quality RNA for sensitive downstream application
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.

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Kit Specifications
Maximum Stool Input	200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed	Preserved, fresh, and frozen stool from humans and animals
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	600 μL
Time to Complete 10 Purifications	30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 49500 (50 preps)
Lysis Buffer C	60 mL
Wash Solution A	38 mL
Elution Buffer E	6 mL
Bead Tubes	50
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Propargyl-PEG2-methylamine is a crosslinker with a propargyl group and methylamine group. The propargyl group forms triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG2-methylamine is a crosslinker with a propargyl group and methylamine group. The propargyl group forms triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.