Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
Detail
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Extract high quality & quantity total RNA including miRNA
No phenol step required – isolate all RNA in one fraction
Rapid processing in under 40 minutes
Isolate total RNA from a wide variety of specimens
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Purification Technology
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells)
~750 μg ~1.5 mg
*For isolating total RNA from purified leukocytes
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Propargyl-PEG14-acid is an alkyne reagent with a carboxylic acid. The carboxylic acid can form amide bonds with amines under the activation of EDC or HATU. The alkyne group can react with azides through Click Chemistry reaction to form triazole linkage. The PEG spacer increases the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG14-acid is an alkyne reagent with a carboxylic acid. The carboxylic acid can form amide bonds with amines under the activation of EDC or HATU. The alkyne group can react with azides through Click Chemistry reaction to form triazole linkage. The PEG spacer increases the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.
Features
Reliable: Rigorous quality control for reproducible separation of protein electrophoresis.
Convenient: Premeasured pouches make 1 liter of 1X buffer solution; No pH adjustment is necessary.
Fast: Dissolving in minutes and then ready to use.
Stable: Powder packaging suitable for long-term storage.
Contents
0.025 M Tris, 0.192 M glycine, 0.10% SDS
Applications
Running buffer for Laemmli Tris-HCl gel electrophoresis
Storage
Room temperature for 24 months
Document
1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.
