Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Detail
Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Accommodates any common 15 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from whole blood using economic salt out method
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Advantages
Safe – without phenol chloroform extraction
Environment friendly – the reagents used are safe, non-toxic and without pollution
High molecular weight – the molecular weight of genomic DNA is about 50-150kb
High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
Unlimited sample size – solution type operation, sample volume can be adjusted at will
Cost performance – the most economical nucleic acid extraction technology
Kit Contents
Contents
D331101
D331102
D331103
Purification Volumes
50 ml
300 ml
1000 ml
10 x Buffer RBC
20 ml
100 ml
3 x 100 ml
Buffer WTL
60 ml
350 ml
1000 ml
Buffer PPS
20 ml
120 ml
350 ml
RNase Solution
300 µl
1.8 ml
6 ml
Buffer TE
10 ml
30 ml
120 ml
Storage and Stability
RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Usages: For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.
Principle: Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is medium coagulant.
Formulation（per liter）: Pancreatic Digest of Casein 10g Beef Extract 5g Yeast Extract 1g Sodium Pyruvate 10g Glycine 12g Lithium Chloride 5g Agar 20g Final pH7.0 ± 0.2
How to use： 1.Suspend 63g in 950ML of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Staphylococcus aureus ATCC6538
Good
black
2
Staphylococcus epidermidis CMCC (B) 26069
Good
Green-blue
3
Escherichia coli ATCC25922
Not growth
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Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Milk samples are stable for 1 month at room temperature (or 1 week at 37°C) in the Preservation Solution
Fast and easy processing using a rapid spin-column format
DNA can be isolated and detected from as little as 100 µL of milk
Isolate high quality genomic DNA
Norgen’s Milk DNA Preservation and Isolation Kit is an all-in-one solution designed for 1) preservation of DNA in milk samples at ambient temperature; and 2) isolation of high quality DNA within a laboratory setting. The kit is provided with a Preservation Solution that allows for either immediate processing of milk samples, or allows for the storage of the milk samples for up to 1 month at room temperature prior to DNA isolation. Genomic DNA extracted from somatic cells and bacteria found in milk can be used in various applications. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Milk DNA can also be used to diagnose particular types of infections. Milk DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Milk DNA Preservative
Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the preservative allow samples to be stored for 1 month without any detectable DNA degradation.
Storage Conditions and Product Stability The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Storage in Preservation Buffer 1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C. 2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality. 3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.
