T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
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T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
Key Features
T7 promoter-specific RNA polymerase
Available in standard glycerol-based formulation as well as lyophilization-friendly formulation without glycerol.
Suggested Applications
In vitro transcription of RNA
Molecular diagnostics (NASBA and other)
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Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. T7 RNA Polymerase is manufactured at our ISO 13485 certified facility in Norway.
Other Products
Boc-Gly-PEG3-endo-BCN
Product Info
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Product Info
Boc-Gly-PEG3-endo-BCN is a Boc protected cick chemistry reagent with a BCN group. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group is reactive with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Boc-Gly-PEG3-endo-BCN is a Boc protected cick chemistry reagent with a BCN group. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group is reactive with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).Benchmarking with products of competitors conducted by us and others show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10.000 wild-type copies straight away without any other tedious assay optimization.Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. (read more) For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
Casestudies: HiDi® DNA Polymerase: Applications from mutation detection to genome editing (read more)
Example Primer Design
Matching vs. mismatching nucleotide is placed at the 3′-end of the primer for best discrimination results.
Example Results – There´s no accounting for taste
Cilantro: some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), “A genetic variant near olfactory receptor genes influences cilantro preference.”)
Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.
Allele-specific PCRs were performed from 1 ng/µl of HeLa gDNA in the presence of a realtime dye, indicating the amplification of the C-allele specific primer only. The A-allele specific primer is discriminated, thus not amplified up to 50 cycles.
PCR products were subsequently analysed on a 2.5% agarose gel. Specific product is visualized by ethidium bromide staining at the amplicon length of 109 bp.
Document
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).
RPA/MIRA is used for DNA and RNA nucleic acid templates isothermal amplification , and can be used in the field of molecular detection of viruses, pathogenic bacteria, tissues, cells, etc.
MIRA VS PCR ,MIRA Advantages:
Low and constant temperature, low requirements on instruments
20mins
Only one pair of primers is needed
Fluorescence detection by probe method is also available
High specificity
PCR :Need to control temperature,90 minutes
LAMP:Design three pairs of primers,60minutes,Low specificity
Document
RPA/MIRA is used for DNA and RNA nucleic acid templates isothermal amplification , and can be used in the field of molecular detection of viruses, pathogenic bacteria, tissues, cells, etc.
