T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
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T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.
Key Features
T7 promoter-specific RNA polymerase
Available in standard glycerol-based formulation as well as lyophilization-friendly formulation without glycerol.
Suggested Applications
In vitro transcription of RNA
Molecular diagnostics (NASBA and other)
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Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. T7 RNA Polymerase is manufactured at our ISO 13485 certified facility in Norway.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Formic Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.4 to 20 µg of formic acid per assay
Limit of Detection:
0.0932 mg/L
Reaction Time (min):
~ 12 min
Application examples:
Wine, fruit juices, pickles, vinegar, jam, bakery products, honey, fish, meat and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by MEBAK
The Formic Acid test kit is a simple method for the rapid, reliable measurement and analysis of formic acid (formate) in foods, beverages and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
No wasted formate dehydrogenase solution (stable suspension supplied)
Pyrazole incorporated to prevent alcohol dehydrogenase interference
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
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The Formic Acid test kit is a simple method for the rapid, reliable measurement and analysis of formic acid (formate) in foods, beverages and other materials.
This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 16/32/48 channel automated extraction system. No need to pause for the addition of binding solution (not applicable to 96/192 instruments)
Details
Kit Contents
Contents
D631001C
D631002C
D631003C
Purification Times
48
96
480
MagPure Particles
1.7 ml
3.5 ml
16 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
3 ml
15 ml
Buffer MLA
30 ml
70 ml
300 ml
Buffer DW1
30 ml
70 ml
300 ml
Buffer EW
60 ml
120 ml
2 x 300 ml
Buffer MWX1
30 ml
70 ml
300 ml
Elution Buffer
10 ml
20 ml
60 ml
Prefilled Kit Contents
Product
Contents and volume
D6310-TL-06C
D6310-S-48
Proteinase K
50 mg
24 mg
Protease Dissolve Buffer
3 ml
1.8 ml
TL-Tip
12 pcs
24 pcs
Tip bottom plate/ Tip base reagent strip
Row 1/7：600μl Buffer MLA
6 plates
48 strips
Row 2/8：600μl Buffer MWX1
Row 3/9：600μl Buffer DW1
Row 4/10：30μl MagPure Particles 600μl Buffer EW
Row 5/11：600μl Buffer EW
Row 6/12：100μl Elution Buffer
Storage and Stability
Proteinase K Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
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This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
A reagent dispensing device which allows a full liter of constant reagent delivery
Permagen Reagent Trough Birdfeeder Kit with Bottle
The Permagen Reagent Trough Birdfeeder Cartridge and Bottle is a device to deliver 1 liter of reagent on the Biomek FX and NX Laboratory Automation Workstations. The unit fits on any standard ALP stand as well
Extended version or Standard available, extended designed to be refilled outside liquid handler, modification to machine may be necessary
Self-leveling fluid line assures the trough will have adequate volume
Hooks onto a Beckman® ALP stand or deck
ANSI/ SBS compliant footprint
Will stand up on bench to refill etc.
Available in white or black trough
Made in the USA
Pull down menu to choose product. Available in Extended version (black or white), standard version in white, or valve only
Please contact us for volume discounts, OEM opportunities, or private labeling.
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A reagent dispensing device which allows a full liter of constant reagent delivery
