T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates. It is also completely inactivated by incubating at 70°C for 10 minutes.
Detail
OverView
T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates. It is also completely inactivated by incubating at 70°C for 10 minutes.
This is a high-quality (commercial grade) version of the T4 DNA Ligase. T4 DNA Ligase is recombinantly produced in E. coli. ArcticZymes’ T4 DNA Ligase is extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA.
Key Features
ATP and Mg2+ dependent dsDNA ligase
Easily heat-inactivated at 70°C for 10 minutes
Extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA
Applications
Ligation of dsDNA
NGS library prep
Molecular cloning
Figures
Other Products
IST-121 QuickSeal qPCR CrystalTM Self Adhesive Sealing Film
Product Info
Document
Product Info
Overview
Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
Optically clear seal specifically developed for optical applications, particularly qPCR
It is non sticky when removed from the packaging; this aids handling when wearing gloves
The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
Document
Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
No phenol extractions
Very sensitive and linear down to a few cells without the need for carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Rapid and convenient spin column protocol
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer.
Salmonella enterica TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Salmonella enterica TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
