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The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
Protocol
The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.
- gDNA is removed, leaving RNA ready for reverse transcription in the same tube (Figure 1).
- Heat-labile dsDNase can easily be inactivated.
- This procedure minimizes pipetting steps and reduces hands-on time.
- The Heat&Run kit is especially well suited for high throughput experiments.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Do you require gDNA removal in applications other than RT-qPCR? Contact our support team for assistance in implementing dsDNase treatment in your workflow.
Kit Contents
- 10X reaction Buffer
- HL-dsDNase (50 or 250 reactions)
