Description 

The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. 

Range 

100 ~ 3,000 bp 

Concentration 

56 µg/ 500 µl 

Recommended loading volume 

5 µl/ well 

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months

The DM2300 ExcelBand™ 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2300 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. 

OverView

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.

The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).

Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

Protocol

The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.

• gDNA is removed, leaving RNA ready for reverse transcription in the same tube (Figure 1).
• Heat-labile dsDNase can easily be inactivated.
• This procedure minimizes pipetting steps and reduces hands-on time.
• The Heat&Run kit is especially well suited for high throughput experiments.

The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.

Do you require gDNA removal in applications other than RT-qPCR? Contact our support team for assistance in implementing dsDNase treatment in your workflow.

Kit Contents

• 10X reaction Buffer
• HL-dsDNase (50 or 250 reactions)

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.

The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).

Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

Introduction

This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit on the amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.

Details

Principles

SolPure DNA Kits is an improved salt precipitation purification method. (Blood samples are lysed in red blood cell lysis buffer to remove red blood cells, and white blood cells are collected by centrifugation.) After lysis, DNA is released into the lysis buffer. RNA is removed by RNASE A. High salt solution is added to precipitate proteins and impurities. Centrifuge to remove precipitate and obtain supernatant containing only DNA. Add isopropanol to precipitate and recover DNA. Wash with 70% ethanol to remove salt, and finally add Buffer TE to dissolve DNA.

Kit Contents

Contents	D3317-01	D3317-02	D3317-03
Purification Times	10	50	250
10 x Buffer RBC	4 ml	50 ml	100 ml
Buffer STE	60 ml	250 ml	2 x 550 ml
Buffer SDS (20%)	6 ml	25 ml	100 ml
Buffer PPS	20 ml	90 ml	400 ml
Proteinase K	12 mg	50 mg	240 mg
Protease Dissolve Buffer	1.8 ml	10 ml	20 ml
RNase A	5 mg	20 mg	60 mg
Buffer TE	10 ml	60 ml	250 ml

Storage and Stability

RNase A and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit on the amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.

Details