For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
Detail
K-TDFC
SKU: 700004345
Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Content:
Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: Ambient, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Dietary Fiber
For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme.
Component
Cat. 13300 (50 preps)
Cat. 46400 (250 preps)
Cat. 46500 (4 preps)
Cat. 46600 (20 preps)
Cat. 60300 (50 preps)
Cat. 63000 (192 preps)
Resuspension Solution AZ
12 mL
60 mL
20 mL
100 mL
12 mL
2 x 20 mL
Lysis Buffer N
40 mL
80 mL
40 mL
2 x 80 mL
40 mL
2 x 40 mL
Buffer TN
20 mL
130 mL
55 mL
2 x 130 mL
20 mL
1 x 55 mL 1 x 20 mL
Wash Solution E
12 mL
2 x 18 mL
–
–
–
–
Elution Buffer K
8 mL
30 mL
–
–
8 mL
2 x 8 mL
Wash Solution J
–
–
25 mL
3 x 25 mL
–
–
Elution Buffer J
–
–
24 mL
120 mL
–
–
RNase A
1 vial
1 vial
1 vial
1 vial
1 vial
1 vial
Magnetic Bead Suspension
–
–
–
–
1 x 1.1 mL
4 x 1.1 mL
Spin Columns
50
250
–
–
–
–
Collection Tubes
50
250
–
–
–
–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)
–
–
4
20
–
–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
α-Amylase, β-Amylase
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.05 U/mL
Reaction Time (min):
~ 20 min (Ceralpha Method), ~ 10 min (Betamyl-3 Method)
Application examples:
Cereal flours, malts, fermentation broths and other materials.
Method recognition:
“Ceralpha” Method: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018). “Betamyl-3” Method: RACI Standard Method
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
Gly-Gly-Gly-PEG4-DBCO is a cleavable PEG linker used in the synthesis of antibody-drug conjugates. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Gly-Gly-Gly-PEG4-DBCO is a cleavable PEG linker used in the synthesis of antibody-drug conjugates. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.