The D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-3-Hydroxybutyric Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
492
Signal Response:
Increase
Linear Range:
0.4 to 12 µg of D-3-hydroxybutyric acid per assay
Limit of Detection:
0.074 mg/L
Reaction Time (min):
~ 6 min
Application examples:
Egg, egg products (e.g. egg powder) and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by EEC
The D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit is suitable for the specific measurement and analysis of D-hydroxybutyric acid in eggs and egg products and other foods and beverages.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 2 years after preparation
Very rapid reaction (~ 3 min)
No wasted diaphorase solution (stable suspension supplied)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Other Products
Heat Labile Exonuclease I
Product Info
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Product Info
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Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
HL-ExoI is used for degradation of ssDNA such as primers and oligos. It is also ideal for treatment of sensitive samples and useful in the development of novel molecular diagnostics applications.
Key Features
3’-5’ exonuclease specific for single stranded DNA
High activity at 25 – 37°C
Easily heat-inactivated by 1 min incubation at 80°C, or 15 min at 60°C
Moderate salt tolerance
Application
Removal of primers post-PCR prior to DNA sequencing or SNP detection
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Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. We manufacture all products at our ISO 13485 certified facility in Norway.
Document
Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
Plasma/Serum Exosome Purification and RNA Isolation Kits
Product Info
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Product Info
Overview
Purification and enrichment of intact plasma/serum exosomes for functional studies
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile plasma/serum input volume
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents, nor overnight incubation required
Compatible with plasma/serum from most species
Pure exosomes are purified and are free from any other RNA-binding proteins
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit
For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit
For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit
For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35 – 40 minutes
Average Yields*
Variable depending on specimen
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
