The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.
Detail
K-RAFGL
SKU: 700004332
120 assays of each per kit
Content:
120 assays of each per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Glucose, Raffinose, Sucrose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Limit of Detection:
100 mg/L
Reaction Time (min):
~ 20 min
Application examples:
Analysis of grain legumes and other materials containing raffinose, stachyose and verbascose.
Method recognition:
Used and accepted in food analysis
The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.
All reagents stable for > 2 years after preparation
Simple format
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
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Product Info
Document
Product Info
Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Accommodates any common 15 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit onthe amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.
Details
Principles
SolPure DNA Kits is an improved salt precipitation purification method. (Blood samples are lysed in red blood cell lysis buffer to remove red blood cells, and white blood cells are collected by centrifugation.) After lysis, DNA is released into the lysis buffer. RNA is removed by RNASE A. High salt solution is added to precipitate proteins and impurities. Centrifuge to remove precipitate and obtain supernatant containing only DNA. Add isopropanol to precipitate and recover DNA. Wash with 70% ethanol to remove salt, and finally add Buffer TE to dissolve DNA.
Kit Contents
Contents
D3317-01
D3317-02
D3317-03
Purification Times
10
50
250
10 x Buffer RBC
4 ml
50 ml
100 ml
Buffer STE
60 ml
250 ml
2 x 550 ml
Buffer SDS (20%)
6 ml
25 ml
100 ml
Buffer PPS
20 ml
90 ml
400 ml
Proteinase K
12 mg
50 mg
240 mg
Protease Dissolve Buffer
1.8 ml
10 ml
20 ml
RNase A
5 mg
20 mg
60 mg
Buffer TE
10 ml
60 ml
250 ml
Storage and Stability
RNase A and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit onthe amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.
