A transparent, optically clear, DMSO resistant, non-tacky film which adheres only when pressure is applied. It is non-pierceable and peelable. Clear plastic, reflective, glossy on the top. Very thin and light and doesn’t crease easily.
Detail
Overview
A transparent, optically clear, DMSO resistant, non-tacky film which adheres only when pressure is applied. It is non-pierceable and peelable. Clear plastic, reflective, glossy on the top. Very thin and light and doesn’t crease easily.
Crystal clear seal specifically developed for optical applications, particularly qPCR
Non-sticky when removed from the packaging aiding with handling when using gloves
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
Other Products
Genomic DNA Extraction Kit (HMW, Magnetic Beads)
Product Info
Document
Product Info
Genomic DNA Extraction Kit (HMW, Magnetic Beads)
The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.
Cat.# 50014 Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads) Cat.# 50015 Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads) Cat.# 50016 Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads)
The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.
Features
High molecular weight DNA: 50 kb to 150 kb
High purity
Simple magnetic beads method
No centrifuge needed
No column needed
No vacuum needed
A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.
Cultured Cell samples
Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Blood samples
Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Tissue samples
Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
All shipping requirements in one convenient mailer
Leak-proof biohazard bags with adsorbent pads for enhanced safety
Matches the standards of OSHA as well as the IATA regulations
Norgen’s Shipping Accessories provides a safe and convenient tool for shipping non-infectious biological specimens. The accessories are contained in individual SHIP TO envelopes that are used for shipping of both the accessories as well as the individual sample collection device to the donor/user.
Each SHIP TO envelope contains:
A Biohazard Specimen Bag with absorbent pad;
A Bubble Envelope labeled as “Exempt Human Specimen”;
2 blank labels;
Shipping Instructions
The Biohazard Specimen Bag is a sure-seal zipper bag to hold the collected specimen and protect against accidental spills. The bag contains an absorbent pad that can adsorb up to 4 mL of liquid and meets current labeling requirements of OSHA standard 29CFR1910.1030 regarding biohazard symbols. The Bubble Envelope is used as a RETURN envelope for the donor/user to ship the collected sample back to the lab for analysis. The user will first place the collected sample into the Biohazard Specimen Bag, and then place the Biohazard Specimen Bag into the Bubble Envelope. The Bubble Envelope protects against moisture and punctures, and facilitates smooth insertion with convenient self-sealing closure. The 2 labels are used for both the outer SHIP TO envelope and the RETURN bubble envelope. The SHIP TO label will be filled out to contain the mailing information of the donor and the RETURN label will be filled out to contain the address to which the sample should be returned. The labels can be supplied as sheets together with the word template file for ease of printing. Norgen’s Shipping Accessories match the regulations of the International Air Transport Association (IATA).
