IST-125 QuickSeal MicroTM Self Adhesive Sealing Film

DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Can be used for the clean up of both RNA and DNA from enzymatic reactions, labeling etc.
• Purifies all sizes of RNA, from large mRNA down to microRNA (miRNA)
• Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA
• Removes endotoxins for transfection of injection ready RNA or DNA
• Rapid and efficient spin-column format (20 minutes)
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications.   The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. 

The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions.

The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.

Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).

Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified.

Details

Supporting Data

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Kit Specifications
Column Binding Capacity	50 μg RNA 10 μg DNA
Maximum Column Loading Volume	600 μL
Minimum Elution Volume	20 μL
Time to Complete 10 Purifications	20 minutes
Size of RNA Purified	All sizes, including small RNA (<200)
Size of DNA Purified	≥ 100 bp*
Average Recovery	≥90% for RNA ≥90% for DNA 100 bp to 10 kbp ≥75% for DNA ≥ 10 kbp

* Applicable to smaller fragments or oligonucleotides with alternative protocol

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

Component	Cat. 23800 (50 preps)
Binding Buffer H	60 mL
Wash Solution K	27 mL
Elution Buffer L	15 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Description

Specifications

Clone	IHC005
Source	Mouse Monoclonal
Positive Control	Tonsil
Dilution Range	1:200

PAX-5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX-5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX-5 is useful in differentiating between classic Hodgkin’s lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin’s lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX-5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX-5.