Brevetoxins (PbTx) are a class of cyclic polyether compounds produced by certain algae such as Karenia brevis. K. brevis can produce Brevetoxins in large quantities during an algae bloom which then pose a major health threat and are important to monitor and mitigate. Brevetoxins are the causative agent of Neurotoxic Shellfish Poisoning (NSP).
FDA and EPA safety levels in regulations and guidance – 0.8 mg/kg for clams, mussels, oysters, and whole and roe-on scallops, fresh, frozen, or canned. – National Shellfish Sanitation Program Guide for the Control of Molluscan Shellfish.
Usages: For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
Principle: Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
Formulation（per liter）: Xylose 3.50g L-Lysine 5.00g Lactose 7.50g Sucrose 7.50 g Sodium Chloride 5.00 g Yeast Extract 3.00g Sodium Desoxycholate 2.50g Sodium Thiosulphate 6.80g Fe-Ammonium Citrate 0.80g Phenol Red 0.08g Agar 13.50 Final PH 7.4±0.2
How to use: 1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes. 2.Dilluted and treated samples.
Quality control: Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows: strain name strain code growth feature Arizona bacteria CMCC (B) 47001 good black colonies Salmonella typhimurium CMCC (B) 50115 good black colonies Streptococcus faecalis CMCC32223 prohibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
