IST-130 QuickSeal qPCR Crystal UltraTM Self Adhesive Sealing Films
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Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.
Detail
Overview
Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.
Crystal clear seal specifically developed for optical applications, particularly qPCR
It is non sticky when removed from the packaging; this aids handling when wearing gloves
The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 1 year under recommended storage conditions
Analyte:
L-Lactic Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.3 to 30 µg of L-lactic acid per assay
Limit of Detection:
0.21 mg/L
Reaction Time (min):
~ 10 min
Application examples:
Wine, beer, soft drinks, milk, dairy products (e.g. cream, milk / whey powder, cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), egg, egg products (e.g. egg powder), baking additives, vinegar, fruit and vegetables, processed fruit and vegetables (e.g. tomatoes), meat products, food additives, feed, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by DIN, GOST, IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK
The L-Lactic Acid (L-Lactate) Assay Kit is used for the specific measurement and analysis of L-lactic acid (L-lactate) in beverages, meat, dairy and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The L-Lactic Acid (L-Lactate) Assay Kit is used for the specific measurement and analysis of L-lactic acid (L-lactate) in beverages, meat, dairy and food products.
This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus multilocularis in human Serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Echinococcus multilocularis Em2-Em18 antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
Biocolor’s Purple-Jelley assay kit is the perfect tool for accurate measurement of hyaluronic acid / Hyaluronan levels in your samples. This colorimetric assay is optimised for quantitative analysis in-vivo, tissue-derived hyaluronic acid / Hyaluronan and includes full step-by-step instructions.
Colorimetric Detection (655nm) (Endpoint)
Hyaluronic Acid: A gentle giant!
Hyaluronic acid, in its hydrated form, is a unique carbohydrate polymer, often referred to as a ‘gentle giant.’ It consists of a lengthy, flexible, non-branching chain with a repeating disaccharide pattern. This disaccharide is composed of alternating uronic acid and aminosugar units.
Why is our kit called ‘Purple-Jelley’?
Discovering the J-Aggregate Effect in Cyanine DyesIn 1936, Edwin Jelley made a fascinating observation, documented it in a letter to Nature (Nature 138, 1009 – 1010). He noted a peculiar behaviour of certain cyanine dyes, that when dissolved in 5 M NaCl, they dyes exhibited a third absorbance peak at a longer wavelength, around 650nm. In deionized water, however, they displayed only a double peak at approximately 540nm and 570nm. The 650nm peak in concentrated dye solutions resulted from the aggregation of dye molecules and was later termed a ‘J-aggregate,’ in honor of Edwin Jelley. The J-aggregate is known as a supra-molecular complex, formed by stacking individual dye molecules.
Subsequent research in the 1960s, notably by Kay et al. (J. Physical Chem. 68, 1896 – 1906), revealed that various biological polymers, including proteins, DNA, polar lipids, and glycosaminoglycans, could also induce this third absorbance peak. This phenomenon led to the development of the Purple-Jelley assay, named after the purple color of the dye reagent and Edwin Jelley himself.
An overview of the Purple-Jelley assay steps:
During the assay, hyaluronic acid is selectively purified during the assay sample preparation protocol. This is then reacted with the Purple-Jelley dye reagent, and the absorption of the characteristic third wavelength recorded. By comparison with a calibration curve the hyaluronic acid content of the sample can be measured.
Step 1. The assay protocol takes tissue samples through a sequential sample preparation protocol which involves enzymatic protein digestion, followed by precipitation and purification of GAGs, culminating in the precipitation of purified Hyaluronic acid.
Step2. The processed sample is then incubated for 10 minutes with the Purple-Jelley dye reagent, forming a coloured product which can be measured spectrophotometrically.
Step 3. The Hyaluronic acid content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising hyaluronic acid (supplied with the kit).
Assay range
10 – 100µg/ml
Limit of Detection
10µg/ml
Detection Method
Colorimetric Detection (655nm) (Endpoint)
Measurements per kit
100 in total (allows a maximum of 46 samples to be run in duplicate alongside a standard curve).
Suitable Samples
In-vivo: Hyaluronic acid purified from in-vivo tissues. The kit protocol involves extraction and purification of hyaluronic acid prior to reaction with the Purple-Dye reagent.
Precautions
This kit is designed for research use only. Not for use in diagnostic procedures. Kit requires access to a centrifuge, as well as a spectrophotometer/colorimeter capable of colorimetric, absorbance detection at 655nm. Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.
2. Hyaluronan Reference Standard (1x 5ml, 0.2mg/ml soluble Hyaluronic Acid)
3. Precipitating Reagent (2x 34ml)
4. Sodium Chloride (1x 20ml)
5. Cetylpyridinium Chloride (1x 20ml)
6. TRIS-buffered Saline (5x tablets)
7. 2ml screw-cap tubes for preparation of samples.
8. Assay kit manual
NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.
Document
Biocolor’s Purple-Jelley assay kit is the perfect tool for accurate measurement of hyaluronic acid / Hyaluronan levels in your samples. This colorimetric assay is optimised for quantitative analysis in-vivo, tissue-derived hyaluronic acid / Hyaluronan and includes full step-by-step instructions.
