Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.
Detail
Overview
Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.
Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
Our ClearASeal Weld is an optically clear polymer film forming a permanent seal to polypropylene plates
The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
The ClearASeal Weld forms a complete seal to a plate, enabling both low and very high temperature uses, including low temperature storage and high temperature incubations
This seal is suitable for PCR/qPCR, even without the use of a pressurized heated lid, and is 100% effective when used in water bath thermal cyclers
The permanent nature of this 100% effective seal renders it suitable for the storage and disposal of hazardous material
ClearASeal Weld demonstrates a good solvent resistance and can be utilized for long term compound storage
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
Programmed Death 1 (PD-1) is a member of the CD28/CTLA-4 family of T-cell regulators, expressed as a co-receptor on the surface of activated T-cells, B-cells, and macrophages. New studies have suggested that the PD-1/PD-L1 signaling pathway may be linked to anti-tumor immunity, as PD-L1 has been shown to induce apoptosis of activated T cells or inhibit activity of cytotoxic T cells. In comparison to CD10 and Bcl-6, PD-1 is expressed by fewer B cells and has therefore been considered a more specific and useful diagnostic marker for angioimmunoblastic T-cell lymphoma. Therapies targeted toward the PD-1 receptor have shown remarkable clinical responses in patients with various types of cancer, including non–small-cell lung cancer, melanoma, and renal-cell cancer.
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
The Animal Disease Kit includes multiple components, such as primers, probes, reagents, buffers, etc., to detect diseases. The kit offers various detection methods, including test strips, making it suitable for a broad range of applications in animal disease detection.
Animal Disease Kit is widely used in cattle veterinary medicine, animal disease detection, and animal feed. The kit has been proved to be reliable and efficient in the detection of animal diseases. It helps to diagnose diseases quickly, providing an accurate basis for subsequent treatments.
Support and Services:
We are committed to providing quality products and services to our customers. Our Animal Disease Kit is designed for veterinary professionals to diagnose and treat medical conditions in animals.
1.Technical Support
Our technical support staff are available to answer any questions you may have about the Animal Disease Kit. We provide assistance with product installation, troubleshooting, and any software or hardware issues you may encounter. We also offer technical advice on how to use the kit and its features.
2.Customer Service
Our customer service team is available to help you with any inquiries you may have about the Animal Disease Kit. We assist with product returns, replacement parts, and warranty information. We also provide product information and advice on how to get the most out of your Animal Disease Kit.
FAQ:
Q1: What is the “Animal Disease Kit” product?
A1: The “Animal Disease Kit” is a comprehensive toolkit for the diagnosis of animal diseases.
Q2: What does the Animal Disease Kit include?
A2: The Animal Disease Kit includes Primers, Probes, Reagents, Buffers, Etc.
Q3: How do I use the Animal Disease Kit?
A3: The Animal Disease Kit is designed for easy and convenient use. You can refer to the instructions manual for detailed instructions on how to use the product.
Q4: How accurate is the Animal Disease Kit?
A4: The Animal Disease Kit is designed for accurate and reliable results. It has been tested and certified by qualified professionals for accuracy and reliability.
Q5: Is the Animal Disease Kit safe to use?
A5: Yes, the Animal Disease Kit is safe to use. All components of the kit are made with non-toxic and non-irritating materials, and all reagents are safe to use.
Document
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
Introducing the Fastin Assay Kit: Your Straightforward Solution for Elastin Quantification! Our user-friendly kit utilizes a dye-based method to measure elastin from in-vivo and in-vitro sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, ‘insoluble’ elastin fibers.
Colorimetric Detection (513nm) (Endpoint)
Understanding Elastin: The Key to Tissue Flexibility
Tissues like lungs and arteries must maintain the ability to stretch and recoil repeatedly throughout an organism’s life. Elastin, a mature protein, is responsible for this elasticity and is usually present as insoluble fibers within the ECM. During development, these fibers are initially formed from a soluble precursor called tropoelastin.
What is the Fastin Assay?
The Biocolor Fastin assay is a user-friendly, dye-based means of quantifying elastins derived from both in-vivo and in-vitro sources. A variety of elastin forms can be assayed, from immature tropoelastin to mature ‘insoluble’ elastin fibres.
Further information on how the assay works can be found on the ‘Mode of Action‘ tab.
A list of suggested sample types can be found under the ‘Assay Specification‘ tab.
How does the Fastin assay detect Elastin?
The Fastin Dye Reagent contains an elastin-binding synthetic porphyrin, TPPS (5,10,15,20-tetraphenyl- 21H,23H-porphine). This affinity of TPPS for elastin was first observed when used as a ‘vital stain’ on live animals. Most tissues took up the dye initially but only elastin retained the TPPS molecules over time. [Winkelman, J. (1962), Cancer Res. 22, 589-596; Winkelman, J & Spicer, S. (1962), Stain Technol. 37, 303-305].
It has been proposed that the elastin binding of TPPS may be due to the retention of the acidic dye (which contains four charged sulfate groups) by the basic amino acid side chain residues of elastin.
How does the Fastin assay work?
Step 1. Incubation of samples containing soluble elastin with the Fastin Dye Reagent causes an elastin-dye complex to form. This insoluble complex then precipiates.
Step 2. Dye-labelled elastin is then isolated by centrifugation and the unbound dye removed. Elastin-bound dye is then eluted and measured spectrophotometrically.
Step 3. The elastin content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising water-soluble elastin (supplied with the kit).
Assay range
50 – 500µg/ml
Limit of Detection
50µg/ml
Detection Method
Colorimetric Detection (513nm) (Endpoint)
Measurements per kit
110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).
Suitable Samples
In-vivo: tissues and fluids. Insoluble elastin will first require conversion to water soluble α-elastin using the oxalic acid reagents and extraction protocol supplied with the kit.
In-vitro: Elastin produced by cells during 2D/3D cell culture. NB elastin in conditioned cell media is typically below the detection limit of the kit.
Precautions
This kit is designed for research use only. Not for use in diagnostic procedures. Kit requires access to a centrifuge, heated water bath or block, as well as a spectrophotometer or colorimeter capable of absorbance detection at 513nm. Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.
Fastin elastin kit contents:
1. Dye Reagent (1x110ml)
2. α-elastin Reference Standard (1x5ml, 1.0 mg/ml soluble Bovine elastin)
6. 1.5ml micro-centrifuge tubes for dye-labelling reaction.
7. Assay kit manual
NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.
Document
Introducing the Fastin Assay Kit: Your Straightforward Solution for Elastin Quantification! Our user-friendly kit utilizes a dye-based method to measure elastin from in-vivo and in-vitro sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, ‘insoluble’ elastin fibers.
Colorimetric Detection (513nm) (Endpoint)
