IST-300 96 Extraction Tube Sealing Film

Introduction

Usages:

For antimicrobial susceptibility testing.

Principle:

Beef extract powder and acid hydrolyzed casein protein provides nitrogen, vitamins and amino acids; soluble starch absorption of toxic metabolites; agar as medium coagulant.

Formulation(per liter):

Agar 17g

Beef extract 2g

Soluble starch 1.5g

Acid hydrolysis of casein 17.5g

Final pH7.3 ± 0.2

How to use:

1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.

2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.

100 count, 200 uL max volume per well

Labware definition is available for immediate use in Opentron’s Labware Library

NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.

100 count, 200 uL max volume per well

Labware definition is available for immediate use in Opentron’s Labware Library

Overview

• CE-IVD marked in accordance with EU Directive 98/79/EC
• Ideal for use in in vitro diagnostic workflows
• Fixative-free preservative, no cross-linking of DNA
• Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C.
• Preserve cf-RNA for 30 days at ambient temperature
• Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
• No plasma volume loss after shipping/transportation
• Prevent hemolysis allowing better separation of plasma
• Prevent apoptosis of blood cells and fragmentation of genomic DNA
• Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
• Vacuumed to draw 8.4 mL of blood in 10 mL tubes
• The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019

Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. 

These tubes are ideal for collecting and preserving cf-DNA and cf-RNA samples for in vitro diagnostic use for medical purposes.

Performance

Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes Dx preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C.

Workflow

NOTE: This product is not available for sale in the United States. For the research use only version, see Cat. 63950

Details

Supporting Data

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Kit Specifications
Tube Size	16 x 100 mm
Tube Type	PET Tube
Blood Draw Volume	8.4 mL in 10 mL Tubes
Preservative Volume	1.6 mL
Recovered Plasma Volume	Approximately 6-7 mL (not affected by shipping)
Anticoagulant	Proprietary
Preservative	Non formaldehyde – Proprietary preservation agent
Length of cf-DNA Preservation	Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C
Length of cf-RNA Preservation	Up to 30 days at room temperature (15-25°C)
Length of CTCs Preservation	Up to 14 days at room temperature (15-25°C)

Storage Conditions and Product Stability

1. When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx are stable through the expiration date.  See tube label for expiry date.
2. Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx.
3. Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
4. Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
5. Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 8 days when stored at 37ºC.
6. Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
7. Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are stable for up to 14 days at room temperature (15-25ºC).

Component	Cat. Dx63950 (50 tubes)
cf-DNA/cf-RNA Preservative Tubes Dx	50
Product Insert	1
Flowchart	1