Working volume of 0.36ml Round well with ‘V’ bottom for maximum liquid extraction High uniformity from well to well Raised well rims for reliable closing with heat sealing. Easy and reliable stacking Good centrifugation stability up to 6,000 × g for faster protocols and improved sample quality Manufactured under DNase/ RNase free environment without slip agents, plasticizers or biocides – Materials which could have a negative effect on bioassays Pure, virgin polypropylene guarantees good extractible performance, high resistance to chemicals, good mechanical stress and working with temperature extremes Autoclavable (121 °C, 20 min)
Detail
Overview
Working volume of 0.36ml
Round well with ‘V’ bottom for maximum liquid extraction
High uniformity from well to well
Raised well rims for reliable closing with heat sealing.
Easy and reliable stacking
Good centrifugation stability up to 6,000 × g for faster protocols and improved sample quality
Manufactured under DNase/ RNase free environment without slip agents, plasticizers or biocides – Materials which could have a negative effect on bioassays
Pure, virgin polypropylene guarantees good extractible performance, high resistance to chemicals, good mechanical stress and working with temperature extremes
Autoclavable (121 °C, 20 min)
Other Products
[CC2102] Champion™ 21, 80 tubes (Region Limited)
Product Info
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Product Info
General information
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Kit contents
Champion™ Competent Cells
pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
Champion™ Transformation Protocol Card
Shipping condition
Throughout the shipping process, the temperature is maintained under -70°C.
Storage and expiration
Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.
Document
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
No sample splitting or need to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Maximum Amount of Starting Material:Animal CellsAnimal TissuesBloodPlant / Fungi Tissues
5 x 105 cells5 mg (for selected tissues)50 µL25 mg
Average Yield* HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells)
RNA: 7.5 μg DNA: 1-2 μg Protein: 70 μg
Storage Conditions and Product Stability The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
β-Glucan
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Limit of Detection:
1 g/100 g
Reaction Time (min):
~ 100 min
Application examples:
Yeast preparations and other materials
Method recognition:
Novel method
This product has been discontinued (see here), please use the recommended replacement product β-Glucan Assay Kit (Yeast and Mushroom) for all your yeast and mushroom β-Glucan testing needs.
Enzymatic Yeast Beta-Glucan test kit, an enzymatic procedure for the measurement and analysis of 1,3:1,6-β-glucan in yeast. Also measures 1,3-β-glucan.
All reagents stable for > 12 months after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Document
This product has been discontinued (see here), please use the recommended replacement product β-Glucan Assay Kit (Yeast and Mushroom) for all your yeast and mushroom β-Glucan testing needs.
