Introduction

Discover Huankai’s Lysine Iron Agar, designed for the differentiation and identification of enteric bacteria, particularly Salmonella, through lysine decarboxylation and hydrogen sulfide production.

Discover Huankai’s Lysine Iron Agar, designed for the differentiation and identification of enteric bacteria, particularly Salmonella, through lysine decarboxylation and hydrogen sulfide prod……

Introduction

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Details

Specifications

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – extraction of several samples can be completed in 60 minutes by column purification method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be purified at the level of PG

Kit Contents

Storage and Stability

Buffer PHC should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolvedbefore use. Make sure that all buffers are at room temperature when used.

Experiment Data

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Overview

Transparent perforated gas permeable film which is peelable, pierceable and suitable for cell culture.

• Our QuickSeal Gas Perm self-adhesive seal is made from an EVA medical tape and is designed for use in cell culture, due to its porous nature
• The seal is compatible with polypropylene, polystyrene, polyethylene and COC
• It can be removed by peeling, or it can be pierced with a pipette tip manually, or using a liquid handling robot.
• QuickSeal Gas Perm can be utilized for effective overnight incubations, during which it demonstrates significant reductions in evaporation compared to lids
• For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer

Transparent perforated gas permeable film which is peelable, pierceable and suitable for cell culture.