This ELISA kit is for the quantitative detection of specific Aspergillus Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).
This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Detail
Name of Product
Aspergillus Antigen (Galactomannan) – ELISA
Catalog Number
ASPE-096
Short Info
This ELISA kit is for the quantitative detection of specific Aspergillus Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).
This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format, TMB, λ=450 nm
Range/Assay Sensivity
Limit of Detection (LOD): 0,5 ng/ml Limit of Quantification (LOQ): 1,0 ng/ml
Test Principle
FungaDia Aspergillus Galactomannan ELISA Detection Kit is a one-step enzyme sandwich microplate immunoassay which detects galactomannan in human serum and BAL fluid. Galactomannan antigen in the sample bind to anti-galactomannan antibodies (sensitized on the microtiter plates) and conjugate (monoclonal antibody/peroxidase). The presence of Galactomannan Antigen is detected with substrate TMB.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 1 year under recommended storage conditions
Analyte:
D-Galacturonic Acid, D-Glucuronic Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
5 to 150 µg of D-glucuronic acid or D-galacturonic acid per assay
Limit of Detection:
~ 15.5 mg/L
Reaction Time (min):
~ 10 min at 25oC or ~ 5 min at 37oC
Application examples:
Hydrolysates of plant material and polysaccharides and other materials.
Method recognition:
Novel method
The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.
Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
Isolate total RNA (including microRNA) without phenol
Isolated RNA is of high quality, integrity and diversity
Also available in 96-well format for high throughput applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
* Yield will vary depending on the type of sample processed.
Kit Specifications – 96-well
Maximum Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified
All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material:FungiPlant Tissues
40 mg40 mg
Average Yield* 40 mg of Grape Leaves 40 mg Tomato Leaves 40 mg Tobacco Leaves 40 mg Peach Leaves
5-7 μg 20-30 μg 20-30 μg 15-20 μg
Time to Complete 96 Purifications
30 minutes
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
