Payment & Shipping Terms

Minimum Order Quantity

48T

Price

USD$3.8/T

Packaging Details

16T/Bag,48T/Box

Delivery Time

6 working days

Payment Terms

T/T, MoneyGram

Supply Ability

100000T/Month

Product Description

MIRA VS RPA,MIRA  Advantages:

1.System optimization and adaptation:MIRA reagents have undergone a series of screenings of the entire system, types and concentrations of cofactors, and multiple optimizations of the production freeze-drying process. Based on the mastery of the components and processes, we can make adjustments according to customer needs to suit them methodology or project.
2.Diversified product forms:For example, we can provide reagents in different systems and forms (dry powder/microsphere/liquid), etc., to achieve personalized customized services according to customer needs.
3.Industrial application support: It has a 4,000-square-meter factory building to support customer projects in entrusting freeze-drying production and industrial application of the project.

MIRA VS RPA,MIRA Advantages:

1.System optimization and adaptation:MIRA reagents have undergone a series of screenings of the entire system, types and concentrations of cofactors, and multiple optimizations of the production freeze-drying process. Based on the mastery of the components and processes, we can make adjustments according to customer needs to suit them methodology or project.
2.Diversified product forms:For example, we can provide reagents in different systems and forms (dry powder/microsphere/liquid), etc., to achieve personalized customized services according to customer needs.
3.Industrial application support: It has a 4,000-square-meter factory building to support customer projects in entrusting freeze-drying production and industrial application of the project.

Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Details

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

1. Suitable for extracting plasmids from 1-5ml or <3ml YT medium.
2. The same amount of buffer 1, 2, and 3 avoids errors caused by adjusting the pipette, making it convenient to use in conjunction with automated workstations.
3. Containing buffer 1 for washing, reducing the problem of false high production.
4. The purified plasmid can be directly used for sequencing, enzyme digestion, PCR, and other applications.

Kit Contents

Storage and Stability

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.

Experiment Data

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Preferred by most scientists, the ring magnet plate is a versatile product that can be used manually or in automated liquid handlers when separating beads into rings is essential.

Available in three magnet strengths like our U500 above

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

S380 / S480 / S500

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• 4 mm bead free ring at bottom of each well for easy removal
• Made in USA 
• For Research use only

Compatibility

• Round bottom plates 
• Pyramid bottom plates 
• PCR Plates
• S380 – Up to 350 µL volumes
• S480 – Up to 1000 µL volumes
• S500 – Up to 2000 µL volumes
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

U380 – Maximum – 350 µL 

Minimum – 30 µL 

U480 – Maximum – 1 mL 

Minimum – 30 µL  

U500 – Maximum – 2 mL

Minimum – 30 µL 

Ideal magnet plate for those wishing to replace Beckman® PN: A32782. (50% Cost Savings*) Features all the same dimensions and even the same magnets. Does not include the spring base but it is the same height. Perfect for those using robots that do not require springs.