HIT – Heparin-Induced Thrombocytopenia Test (20)

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Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

Detail

Name of Product

HIT – Heparin-Induced Thrombocytopenia Test (20)

Catalog Number

MQHIT 1

Short Info

Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

This product is only available in Germany!

Method/Platform

lateral flow, immunoassay

Range/Assay Sensivity

Test Principle

IgG antibodies are resposible for the heparin induced thrombocytopenia.

Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.

The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.

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High yield and high purity DNA ready for any application
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Compatible with blood collected on a variety of commercially available tubes

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This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

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Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 19

Figure 1. The difference of Ct values obtained from a Taqman qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants

Figure 1. The difference of Ct values obtained from a Taqman qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants

Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit.

Figure 5. High Yields of DNA

Figure 6. High Quality DNA

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants

Figure 16. High Yield of Blood DNA

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue)

Figure 18. DNA Isolated from Blood Preserved in EDTA Anticoagulants

Figure 19. Detection of GAPDH From Blood Samples Preserved in EDTA Anticoagulants

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Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1

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