HIT - Heparin-Induced Thrombocytopenia Test (20)

20 tests

Name of Product

HIT – Heparin-Induced Thrombocytopenia Test (20)

Catalog Number

MQHIT 1

Short Info

Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

This product is only available in Germany!

Method/Platform

lateral flow, immunoassay

Range/Assay Sensivity

Test Principle

IgG antibodies are resposible for the heparin induced thrombocytopenia.

Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.

The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.

Kit Specifications – Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi	50 mg 1 x 10⁶ cells 50 mg (wet weight)
Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves	60 μg 60 μg 32 μg 35 μg 30 μg
Time to Complete 10 Purifications	30 minutes

Component	Cat. 25800 (50 preps)	Cat. 31350 (100 preps)	Cat. 25850 (250 preps)	Cat. 31900 (192 preps)
Lysis Buffer C	60 mL	1 x 30 mL, 1 x 60 mL	3 x 60 mL	2 x 60 mL
Wash Solution A	38 mL	38 mL	1 x 18 mL, 2 x 38 mL	2 x 38 mL
Elution Solution A	6 mL	6 mL	20 mL	20 mL
Filter Columns	50	100	250	–
Spin Columns	50	100	250	–
96-Well Plate	–	–	–	2
Adhesive Tape	–	–	–	4
Collection Tubes	100	200	500	–
96-Well Collection Plate	–	–	–	2
Elution Tubes (1.7 mL)	50	100	250	–
96-Well Elution Plate	–	–	–	2
Product Insert	1	1	1	1

Content:	100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	D-Galacturonic Acid, D-Glucuronic Acid
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	5 to 150 µg of D-glucuronic acid or D-galacturonic acid per assay
Limit of Detection:	~ 15.5 mg/L
Reaction Time (min):	~ 10 min at 25^oC or ~ 5 min at 37^oC
Application examples:	Hydrolysates of plant material and polysaccharides and other materials.
Method recognition:	*Novel method*

HIT – Heparin-Induced Thrombocytopenia Test (20)

Detail

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Plant/Fungi Total RNA Purification Kit

Overview

Details

Supporting Data

Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits

Plants

Fungi

D-Glucuronic/D-Galacturonic Acid Assay Kit

A3P Scientific

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