HybriDetect Cassette

Name of Product

Aspergillus Antigen
(Galactomannan) – Rapid Test

Catalog Number

ASP-025

Short Info

This Rapid Test is for the detection of specific Aspergillus Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).

This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

Immunochromatographic Rapid Test

Range/Assay Sensivity

Test Principle

The principle of the test is colloidal gold immunochromatography. If the sample is positive, the antigens in the sample react with the red-colored nanoparticles and form a complex (Antigen – anti-Aspergillus monoclonal antibodies – gold nanoparticles), which was previously pre-dried on the conjugate pad. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the binding conjugate complexes migrate. The anti-Aspergillus antibodies present on the membrane (Test Line) capture the colored conjugate complex and a red line will appear.

Brief Instructions

Sample Preparation and Result Interpretation

Storage

2-8° C

Components

Aspergillus Galactomannan Detection Test, Positive Control, Negative Control, Sample Treatment Solution

25 tests
This Rapid Test is for the detection of specific Aspergillus Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).

This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Propargyl-PEG5-CH2CO2H is an alkyne linker with a carboxylic acid. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-CH2CO2H is an alkyne linker with a carboxylic acid. The carboxylic acid can react with primary amines to form stable amide bonds; activator (e.g. EDC, or HATU) is needed. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Product Description

Highly Sensitive Isothermal Amplification Kit with Fast Reaction Time

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.