Diagnosis Nucleic Acid Detection White Spot Syndrome Virus Livestock Disease Kit

Character

Yingtai   pilot-type   freeze   dryer   completely   simulates   the   technical   requirements   of  the   industrial   freeze   dryer,with   a   compact   and   reasonable design,a   large   redundancy   range,and   conforms   to   GMP  and   FDA   standards.The   ice   condensation   capacity   is   4~18kg,and  the  WRL  controller and   liquid   temperature   control   shelf   are   adopted,so   that   the    process   of   pre-freezing   and   freeze-drying   can    be   precisely   controlled   on   the main   machine,and   the    process   results    are   quite   comparable   with   the    production   machine.In    addition   to    supporting   the   eutectic    point   test system,pressure    rise    and     pressure    comparison     method    to    determine     the    freeze-drying    end     point    and     freeze-drying    curve    recording software,hallan   pilot-type   freeze    dryer   can   also    be   equipped   with    engineering   WRL   control   software   to   directly    Graphical   computer   control the   freeze-drying   process.It    can   be    configured   with    H202 sterilization    and   high-pressure    steam   sterilization    orintegrated   glove    box   system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel  to  ensure  the  cleanliness  of  the  chamber  and  good   ultimate  vacuum  and  vacuum   leakage  rate  to  the  greatest  extent.

Refrigeration Series

●  Dual  compressors,Secop  special   compressors,air  cooling (water  cooling  system  is  optional)

●   Refrigeration   method:Cascade   refrigeration    method,high   cooling efficiency,good   reliability,using   CFC-Free   environmentally   friendly refrigerant.

Silicone oil conducts heat,with uniform temperature and accuracy up to  0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.

● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.

Vacuum   series

●      Vacuum      pump:German       Pfeiffer      anti-corrosion,anti-oil      return       function vacuum   pump.(Vacuum    pump   can    be   selected    according   to    user   needs)

●       Pumping        rate:9m³/h~62m³/h,multiple        specifications       are        optional,the ultimate  vacuum  can  reach  2×10-3mbar,and  the  oil  mist  filter  is  standard.

●   The   vacuum    pipelines    are   all    high-vacuum   stainless    steel   vacuum    tubes, which  can  effectively  prevent  vacuum  leakage  and  corrosion.

●   The    double   anti-oil    return    device    completely   eliminates    the    damage   and

equipment   and   samples.

●    Automatic    backfill    air    device,the    system     has    a    built-in     High    efficiency particulate  air(HEPA)filter  to  ensure  the  complete  cleanliness  of  backfill  air.

●   The   vacuum   stabilization   system    can   stabilize   the   vacuum    degree   of   the freeze-drying    process    through     the    micro-pass    vacuum    control    valve    to ensure  that  the  samples  will   not  be  affected  by  excessive  fluctuations   in  the vacuum    degree.

●   Condensed    liquid   collection   and   discharge   system,automatically   completes the   collection   and   discharge   of   condensed   liquid   after   freeze-drying.

Control    System Characteristic

●               Control:Automatic/Manual

●   Can   store    at   least    100    independent   freeze-drying    programs,each   program    contains   32    program   segments    (Can   be

customized   according    to    user    needs),each   program   segment    corresponds    to   a    processing    stage    (pre-freezing,main

drying,post-drying).and    Include     the    following     segment    values:Cycles,shelf     temperature,vacuum    and     safety    pressure

●  All  the  equipment  can   be   recorded   in   real   time   and   the   corresponding   freeze-drying   curves   and   data  tables   can   be generated  after  the   end.The  records  of  all   data  and  curves  comply  with   the  corresponding  regulations  and  standards.

●    It    has    hierarchical    management    authority,hierarchical    management    allocation    authority    and    usage    process    tracking authority

● Remote    System

O   Fully   support   2G/4G/GSM/Wi-Fivarious   connection    modes

o  Users can remotely view all data on the device and operate it

o  Technicians  can  remotely  perform  maintenance  and  fault  detection  on  equipment,and  can  remotely  guide  and  train  users

o  Can  remotely  upgrade  and  change  the  system  of  the  equipment

Parameter

Mode	MP2	MP5	MP8	MP12
Freeze-dried area	0.24m	0.48m²	0.96m²	1.45m²
Ice condenser capacity	4kg	9kg	12kg	18kg
Shelf temperature	70℃~+80℃			65℃~+70℃
Ice condenser temperature	70 /-90℃	70/-90℃	-70℃/-90℃	-70℃/-85℃
Cold trap volume	9L	15L	18L	30L
Chamber type	single	double
Max number of shelves	2+1	5+1	8+1	12+1
Shelf size	275*400 mm	275*400 mm	365*465 mm	365*465 mm
Shelf spacing	100mm(adjustable)	100mm(adjustable)	70mm(adjustable)	70mm(adjustable)
Sample quantity (4R Cillin bottle)	558	1395	2630	4230
Shelf heating and cooling method	Thermal conducting silicone oil medium(temperature resistance:-100℃~+600℃)
Chamber and shelf surface	Atomization and electrolytic surface treatment,no dead corner residue
Pipeline diameter		中 8 9 m m
External valve port	6/	12/	18/
Equipment power	3.8kw	5.5kw	6.5kw	8kw
Equipment size	700*588*1600 mm	1030*700*1630  mm	1030*700*1850 mm	1500*850*1900 mm
Capping mode	Hydraulic system gland device/no gland			Hydraulic pressure
Suitable clean room	suitable
Vacuum sensor type	PiraniVacuum sensor/capacitive vacuum sensor
Remote control and alarm	Support 2G/4G/GSM/Wi-Fi
Terminal judgment function	Pressure contrast method         Pressure test/pressure contrast method
Freeze dryer front door cover	Stainless steel/Plexiglass			Stainless steel
Inert gas filling	(Optional)Sufficient amount and time for the program to run itself
User function customization	Can be customized and modified according to user needs
Sterilization method	HzOz/steam sterilization
Note:”/”optional.Pilot models can be customized according to user needs

Description 

The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)

Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control 

Under suggested conditions, PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range resolves 13 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months 
-20°C for long term storage

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference band at 500 bp

The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for fast running times and accurate visual determination.

Contents:

1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

Figure 1 / 1

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LowRanger 100bp DNA Ladder (Cat# 11500) – 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 100 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference.

Fragment	Size (bp)	Mass (ng)
1	2000	110
2	1500	83
3	1000	55
4	800	44
5	700	39
6	600	33
7	500	55
8	400	22
9	300	17
10	200	22
11	100	22

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.