DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Perfect for:
• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQBAS01
K-FRHYD
SKU: 700004284
50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
| Content: | 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Formaldehyde |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.1 to 14 μg of formaldehyde per assay |
| Limit of Detection: | 0.033 mg of formaldehyde per test or 0.016 mg/mL of formaldehyde in a sample treated as per the standard procedure |
| Limit of Quantification: | 0.11 mg of formaldehyde per test or 0.054 mg/mL of formaldehyde in a sample treated as per the standard procedure |
| Reproducibility (%): | ~ 3% |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Environmental samples, food and beverage samples. |
The Formaldehyde Assay Kit provides a simple robust method for the measurement of formaldehyde.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Display all assay test kits available.
Advantages
The Formaldehyde Assay Kit provides a simple robust method for the measurement of formaldehyde.
Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA within a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components:
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed, the Collection Tube is securely capped with Collection Tube Cap. The collected saliva is mixed by gentle inversion for several times till the saliva colour changes into yellow. The Saliva Collection Tube is subsequently sent to the laboratory for the purification of intact exosomes and for the subsequent isolation and analysis of saliva exosomal RNA. The saliva exosomes in preserved samples are stable for 2 years at room temperature.
| Kit Specifications | |
| Volume of Saliva Collected | 2 mL |
| Preservation Temperature | Room Temperature |
| Preservation time | 2 years at room temperature |
Shelf Life and Handling
| Kit Components | Cat. 65400 (50 Devices) |
|---|---|
| Individual Saliva Exosome Collection and Preservation Devices | 50 |
| Donor Procedure Flowchart | 1 |
| Product Insert | 1 |
| Individual Saliva Exosome Collection and Preservation Device Contents | |
| Saliva Collection Funnel and Collection Tube | 1 |
| Collection Tube Cap | 1 |
| Donor Instructions | 1 |
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
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DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications. DNA size selection is preferred after NGS library prep in most of the cases. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
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A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
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Tel : 081-875-1869 , 02-328-7179
Email : [email protected]
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