Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.
Detail
Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.
Isotherm3G exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.
Isotherm3G DNA Polymerase utilizes an aptamer-based warm-start feature to prevent false amplification at low temperatures. For more information, please contact us.
The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation.
Other Products
TwistAmp® Liquid Basic
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Product Info
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Perfect for:
• End-point gel electrophoresis DNA detection • down-stream applications (e.g. sub-cloning) • facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQBAS01
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DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Micronucleic acid extraction and purification, virus total nucleic acid extraction
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/F, 3 layers
Membrane aperture
0.7μm
Maximum binding yield of plasmid
10 μg
Maximum yield of alcohol mediated Binding
50 μg
Single liquid carrying capacity of column
700 μl
Minimum elution volume
15 μl
Withstand centrifugal force
12,000 x g
Centrifuge
Small high speed centrifuge (2ml)
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13011
HiPure DNA Micro Column I (3 x GF/F)with 2ml Collection Tubes
1000/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
Staphylococcus aureus Quantified Bacterial DNA Standard
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Overview
Quantified standard to be used as a positive control or PCR quantification standard
Vigorously quantified using multiple methods
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus (S. aureus) is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry. S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulase-positive, which forms the basis of traditional identification methodology.
Staphylococcus aureus Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Staphylococcus aureus.
Upon receipt, store Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
